Trf Scanning - Thermo Scientific Varioskan User Manual

TRF scanning

Thermo Fisher Scientific
• Add - Enter the new measurement parameters in the Ex [nm], Em [nm],
Delay time [µs] and Integration time [µs] boxes and then click Add. The
new value pair is added to the list on the left.
• Remove - Select the measurement parameters you want to remove from
the list above and click Remove.
• Set - The parameters of the currently selected measurement are
substituted with the values defined on the left.
Click OK to close the dialog and add the new measurements to the step
parameters.
• Measurement interval [hh:mm:ss.t] - Define the time between
measurements with different wavelengths.
In the TRF Scanning step, either the emission or the excitation wavelength
is fixed and the other wavelength is scanned.
Select Steps > TRF Scanning or click the
Thermo Scientific SkanIt
button on the action panel.
® ®
Software for Varioskan User Manual
Step Parameters
TRF scanning
107