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Data Processing Parameters
Blank subtraction
Examples of blank
subtraction
148
Thermo Scientific SkanIt
Blanks are positioned in the first well on both plates:
Plate 1
0.01
0.3
0.4
0.5
Plate 2
0.02
0.35
0.45
0.55
Example 10-1 Average blank subtraction
The blank average is: (0.01 + 0.02)/2 = 0.015
The blank subtracted data would then be (with an accuracy of three
decimals):
Plate 1
-0.005
0.285
0.385
0.485
Plate 2
0.005
0.335
0.435
0.535
®
®
Software for Varioskan
User Manual
Thermo Fisher Scientific
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