Thermo Scientific Varioskan User Manual page 131

Thermo Fisher Scientific
• Type - Select the type of sample from the drop-down list. The available
types are Blank, Calibrator, Control, Empty and Unknown.
Note If you want to create several calibrators with the same
concentration, it is recommended to create one calibrator and then
create an appropriate number of replicates of the calibrator by using
the No. of replicates setting. Otherwise the cubic spline as a fit
type in the quantitative curve fit calculation will not process
correctly.
• Name - The name is automatically suggested. The name is used in the
calculations. If the value of No. of <samples> is more than one, the
names are given running numbers, for example, Un_0001, Un_0002,
and so on. You can change the name. You can also start the numbering
from a number of your own choice by writing the name and an
underscore (_) followed by the desired starting number, for example,
Un_0100. The samples will be named Un_0100, Un_0101, etc.
• Group - The name of the sample group. The default group is Assay, but
other groups may be defined by entering a new group name in the Group
field. You can enter several groups for a sample in the field by separating
the groups with commas.
• No. of <samples> - Enter the number of samples of the selected type.
Not available for Blanks.
• Filling order - Select in which order the samples are added to the plate
layout.
Replicates:
• No. of replicates - Enter the number of replicates for each sample of
the selected type. The number of replicates can vary from one (1) to the
number of wells on the plate (i.e., 96 on a 96-well plate, and so on).
• Filling order - Select in which direction the replicates are added to the
plate layout.
• Specific blanks - Select the number of specific blanks (individual sample
blanks) in use: none, one or two. If you select one or two specific blanks,
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Plate Layouts
Editing a plate layout
129