Trf Decay Calculation - Thermo Scientific Varioskan User Manual

TRF decay calculation

Thermo Fisher Scientific
The TRF Decay Calculation step is used to calculate the t (tau) and a (alpha)
values based on the data from a TRF (time-resolved fluorescence) decay
measurement. The tau value is the half-life of the TRF label signal. The
alpha value is the peak of the measured signal. The correlation coefficient
2
R shows the validity of the signal; it should be as close to 1 as possible.
The tau and alpha values are calculated using the following formula as a
starting point:
The t (tau) value is then rounded to the nearest µs.
The Recommended integration time (RIT [µs]) is calculated using the
formula below:
The RIT value is then rounded to the nearest µs.
The TRF Decay measurement can produce a high peak in the first few
measurements if the background fluorometric decay is slow and the start
time for TRF Decay is too low. You can disable the first few measurements
from the raw data if the peak seems to be too high. This will ensure that
you get the best possible fit for the data. For information on how to disable
measurements, see Right-click menus" on page 50.
Select Data Processing > TRF Decay Calculation, click the
the Results action panel, or right-click the measurement in the Results tree.
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Software for Varioskan User Manual
TRF decay calculation
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