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Measurement with
dispensing
Thermo Fisher Scientific
•
Type - Well
•
Well count - 1
•
Execution order -
The instrument performs the execution as follows:
1.
The instrument dispenses into well A1 and measures A1 with the
fluorometric parameters as many times as defined in the kinetic loop.
2.
The instrument drives the plate to position B1.
3.
The instrument dispenses into well B1. Well B1 is measured with the
fluorometric parameters as many times as defined in the kinetic loop.
4.
The instrument drives the plate to position C1.
5.
The well loop is thus executed until all the wells are measured.
When the setting Type is changed from Well to Plate, the execution order
is as above. However, if only dispense and measurement steps are used in
the well loop without the kinetic loop, the execution order is as follows:
1.
All the wells are dispensed (in the order A1, B1, C1, D1, D2, C2, B2,
..., B6, A6).
2.
The instrument returns the plate to the original position.
3.
Well A1 is measured with the fluorometric parameters.
4.
The instrument drives the plate to position B1 and measures B1.
5.
The well loop continues until all the wells are measured once.
6.
The instrument returns to position A1 and measures A1 again, then
well B1, and so on.
7.
The wells are thus measured as many times as defined in the kinetic
loop.
In fast kinetic measurements, it is often necessary to start the measurement
of the sample immediately when the dispensing is started, not when the
dispensing has been completed. Figure 6-2 shows a measurement with
dispensing. The delay between the start of dispensing and the start of
measurement is so small that no liquid has yet reached the well when the
Thermo Scientific SkanIt
Measurement with dispensing
®
®
Software for Varioskan
User Manual
Protocols
89
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