Blank Subtraction In Kinetic Measurements - Thermo Scientific Varioskan User Manual

Blank subtraction in kinetic
measurements
Thermo Fisher Scientific
Example 10-2 Plate blank subtraction
The blank subtracted results calculated from the same raw data would be
(with an accuracy of two decimals):
Plate 1
0.00
0.29
0.39
0.49
Plate 2
0.00
0.33
0.43
0.53
You can use all modes of blanking in kinetic measurements as well. Normally
this means that the kinetic data is first reduced to rates, peak maximums,
integrals, and so on by using kinetic calculations (see Kinetic calculations"
on page 183), and then the blank subtraction is performed to the reduced
data.
Consider, for example, a kinetic measurement with a plate containing one
blank, the rest being other samples, and an average rate calculation with
linear regression. The rate is calculated separately for all samples. The reduced
rate is then calculated by subtracting the rate of the blank sample from the
rates of other samples.
Alternatively, you may specify point-wise kinetic blank reduction directly
from measurement data. In this mode, the raw blank signal at each time
point is subtracted from raw sample signals at that specific time. This
produces reduced curves for all samples. Only then is the kinetic processor
applied to the reduced curves to determine the reduced rate, and so on.
Note If a certain rate cannot be determined, for example,
increasing maximum rate if the whole reaction is decreasing, the
software returns NaN (not a number).
Thermo Scientific SkanIt
Data Processing Parameters
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Software for Varioskan User Manual
Blank subtraction
149