Baseline Subtraction; Figure 10-16 Determining The Average Value Of A Kinetic Measurement - Thermo Scientific Varioskan User Manual

Data Processing Parameters
Kinetic calculations

Baseline subtraction

196 Thermo Scientific SkanIt
Figure 10-16. Determining the average value of a kinetic measurement
You can define the following settings:
• Ignore first (n) readings - Ignores a defined number of points
(readings), counted from the first reading.
• Ignore last (n) readings - Ignores a defined number of points (readings),
counted from the last reading.
Baseline subtraction is used to transform the measured values to start from
0 by removing the measured baseline before the actual reaction. It subtracts
the average of a defined number of readings from the beginning or the end
of all other readings. This is useful when used with a kinetic loop with
dispensing and measurement. For example, you can set the dispensing to
occur in reading 5 to obtain 4 measurements from the non-reactive state
and then do a baseline subtraction to ensure that the reaction in all wells
starts from 0.
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