Maximum Rate; Figure 10-12 Determining The Maximum Rate With Window Value 2 - Thermo Scientific Varioskan User Manual

Maximum rate

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• Ignore last (n) readings - Ignores a defined number of points (readings),
counted from the last reading.
If the maximum rate is selected, the software searches the data for the
maximum rate found in each well. To obtain the maximum rate, a series of
linear curve fits will be performed for different segments of the measurement
value, absorbance or fluorescence intensity vs. time curve (Figure 10-12).
The first segment starts at the first data point within the selected time and
measurement range, the second segment starts at the second data point, and
so on, until all the data points have been analyzed. All the rate calculations
are evaluated to determine the maximum rate. In other words, the LLS fit
of m span points are sequentially fitted through each of the n data points.
There will be n - m + 1 curves produced from this. You can specify the
number of data points in a segment with the Window (2...100) setting.
Figure 10-12. Determining the maximum rate with window value 2
You can define the following settings:
Thermo Scientific SkanIt
Data Processing Parameters
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Software for Varioskan User Manual
Kinetic calculations
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