Thermo Scientific Varioskan User Manual page 106

Step Parameters
TRF measurement
104 Thermo Scientific SkanIt
• Excitation wavelength [nm] - Select the excitation wavelength to be
used in the measurement. The wavelength range is 200 to 840 nm; the
default value is 340 nm.
• Emission wavelength [nm] - Select the emission wavelength to be used
in the measurement. The wavelength range is 270 to 840 nm; the default
value is 615 nm.
• Excitation bandwidth [nm] - Select the bandwidth of the excitation
wavelength to be used in the measurement. Bandwidth is selected using
an excitation slit that has 5 nm and 12 nm options. The default value
is 12 nm. The emission bandwidth is permanently set to 12 nm and
cannot be changed.
Note To prevent any stray light from the excitation light from
interfering with the emission light, the difference between the
excitation and emission wavelengths should be at least the sum of
the bandwidths used. If the 5 nm excitation and 12 nm emission
(permanent) bandwidths are used, the difference between excitation
and emission wavelengths should be at least 17 nm. With the 12 nm
excitation and emission bandwidths the difference should be at
least 24 nm.
• Dynamic range - Select the dynamic range to be used in the
measurement: AutoRange, Low, Medium Low, Medium High, or High.
For more information, see Dynamic range selections" on page 112.
• Measurement time [ms] - The measurement or integration time defines
the light collection time used in the measurement. The wells are
measured with a selected measurement time. For more information, see
Measurement times" on page 113.
• Lag time [hh:mm:ss.t] - Define the time before the measurement is
executed. Lag time is not available for kinetic loops.
• Step duration [hh:mm:ss] - Define the total time for the step. The
software waits after the instrument has performed the action until the
time is up before proceeding with the following step. Step duration is
not available for kinetic loops.
• Multipoint - Tick the check box to select which points in a well are
measured. If the multipoint measurement is not selected, only the center
of the well is measured. Multipoint is only available for 6 to 48-well
plates. For more information, see Using multipoint in a measurement"
on page 114.
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Software for Varioskan User Manual Thermo Fisher Scientific