Thermo Scientific Varioskan User Manual page 8

Contents
Chapter 5
Chapter 6
Chapter 7
6 Thermo Scientific SkanIt
Moving folders or sessions ................................................................. 57
Renaming folders .............................................................................. 57
Deleting folders ................................................................................. 58
Opening an existing session ................................................................. 59
Creating a new session ......................................................................... 61
Editing a session .................................................................................. 63
Saving a session .................................................................................... 63
Deleting a session or a run from the database ....................................... 64
Exporting and importing sessions ........................................................ 65
Exporting sessions or runs ................................................................. 65
Importing sessions or runs ................................................................. 66
Executing a Session ............................................................................ 69
Pathlength correction .......................................................................... 72
Measuring new pathlength correction K-factors ................................ 73
Priming dispensers ............................................................................... 75
Emptying dispensers ............................................................................ 76
Washing dispensers .............................................................................. 77
Example of dispenser washing ........................................................... 78
Protocols ............................................................................................. 81
Editing a protocol ................................................................................ 81
Adding new protocol steps ................................................................ 83
Deleting protocol steps ...................................................................... 85
Area definition ..................................................................................... 85
Well loops ........................................................................................... 87
Measurement with dispensing .............................................................. 89
Kinetic loops ....................................................................................... 91
Step Parameters .................................................................................. 93
Area definition ..................................................................................... 93
Well loop ............................................................................................. 93
Photometric measurement ................................................................... 93
Photometric spectrum scanning ........................................................... 95
Using pathlength correction in a measurement .................................... 97
Fluorometric measurement .................................................................. 99
Defining multiple fluorometric measurements ................................ 101
Fluorometric spectrum scanning ........................................................ 101
TRF measurement ............................................................................. 103
Defining multiple TRF measurements ............................................ 106
TRF scanning .................................................................................... 107
TRF decay ......................................................................................... 109
Dynamic range selections ................................................................... 112
Measurement times ........................................................................... 113
Using multipoint in a measurement ................................................... 114
Kinetic measurements ........................................................................ 116
Incubate ............................................................................................ 116
Shake ................................................................................................. 117
Shaking speed and diameter combinations ...................................... 119
Dispense ............................................................................................ 120
Pause ................................................................................................. 122
® ®
Software for Varioskan User Manual Thermo Fisher Scientific