Thermo Scientific Varioskan User Manual page 96

Step Parameters
Photometric measurement
Defining multiple
photometric measurements
94 Thermo Scientific SkanIt
• Wavelength [nm] - Select the wavelength to be used in the
measurement. The wavelength range is 200 to 1,000 nm; the default
value is 450 nm.
• Bandwidth [nm] - The bandwidth value in a photometric measurement
is 5 nm.
• Lag time [hh:mm:ss.t] - Define the time before a measurement is
executed. Lag time is not available for kinetic loops.
• Step duration [hh:mm:ss.t] - Define the total time for the step. The
software waits after the instrument has performed the action until the
time is up before proceeding with the following step. Step duration is
not available for kinetic loops.
• Measurement time [ms] - The measurement time defines the light
collection time used in the measurement. The wells are measured with
a selected measurement time. For more information, see Measurement
times" on page 113.
• Pathlength correction - Tick the check box to launch the Pathlength
Correction K-Factors dialog. Select the pathlength correction factor
for the solvent you are using (see Using pathlength correction in a
measurement" on page 97).
• Multipoint - Tick the check box to select which points in a well are
measured. If the multipoint measurement is not selected, only the center
of the well is measured. Multipoint is only available for 6 to 48-well
plates. For more information, see Using multipoint in a measurement"
on page 114.
• Multiple measurements - Tick the check box to select multiple
wavelengths for measurement. All wavelengths are measured one after
the other from the same well before moving to the next well. For more
information, see the section called Defining multiple photometric
measurements".
Note In the photometric calibration procedure the instrument
reads the air blank level. In long measurement procedures
calibration is performed in a suitable phase without disturbing the
measurement timing. The calibration is valid for 1 hour.
When you select the Multiple Measurements check box while defining
the photometric measurement parameters, the following dialog opens:
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