Thermo Scientific Varioskan User Manual page 107

Thermo Fisher Scientific
Note If there are not any points selected in the multipoint
measurement, the protocol cannot be run.
• Optics - Fluorescence can be measured from one position:
• Top - Fluorescence is measured at the measurement position above
the plate.
• Bottom - This option is available with the Varioskan Flash only.
• Multiple measurements - Tick the check box to enter a multiple
wavelength pair and TRF parameter values. For more information, see
Defining multiple TRF measurements" on page 106.
• TRF delay time [µs] - Define the waiting period (TRF delay) between
the excitation flash and the beginning of the emission light measurement
(see Figure 7-1).
• TRF integration time [µs] - Define the length of the measurement
window, that is, the time the emitted signal is collected for each
measurement cycle (see Figure 7-1).
The sum of TRF delay time and TRF integration time can be maximum
2400 µs.
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Step Parameters
TRF measurement
105