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Ratio Within Spectrum; Figure 10-10 Intensities I And I In Ratio Within Spectrum - Thermo Scientific Varioskan User Manual

Multimode microplate reader software

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Ratio within spectrum

Thermo Fisher Scientific
Control - Select the sample/replicate for which the calculation is
performed.
Ratio within spectrum calculates the ratio of intensities for two different
wavelengths within the same spectrum.
Two wavelengths, n
and n
A
given. For each spectrum, the ratio of the intensities for the given
wavelengths is calculated according to the formula given below. The result
is displayed in a matrix.
Figure 10-10. Intensities I
Wavelength 1 - Select the wavelength of the first peak, I
Wavelength 2 - Select the wavelength of the second peak, I
Thermo Scientific SkanIt
, corresponding to the intensities I
B
and I
in ratio within spectrum
A
B
®
Software for Varioskan
Data Processing Parameters
Spectral analysis
and I
, are
A
B
.
A
.
B
®
User Manual
181

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