Hbw Calculation; Figure 10-6 Definition Of The Hbw Value - Thermo Scientific Varioskan User Manual

Data Processing Parameters
Spectral analysis

HBW calculation

178 Thermo Scientific SkanIt
• Threshold - Enter the intensity threshold that the peak is required to
exceed. The minimum value is –10,000,000 units and the maximum
is 10,000,000.
In HBW calculations, a certain peak is identified and its intensity is
measured. The calculation analyses the shape of the curve between the 100%
(maximum) and the 50% values. The wavelengths on both sides of the peak
corresponding to 50% of the peak intensity are identified and the difference
between these two wavelengths is calculated. This is the HBW value. The
result of this calculation will be the HBW value and the peak wavelength
(Figure 10-6).
The HBW can only be calculated for one well at a time.
The peak value should first be identified using either spectral peak search
(see Spectral peak search" on page 174) or spectral maximum (see Spectral
maximum" on page 176).
Figure 10-6. Definition of the HBW value
If the 50% intensity value is not found from either lower or higher
wavelengths, the calculation cannot be performed. See the following
examples:
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