Thermo Scientific Varioskan User Manual page 81

Thermo Fisher Scientific
Before you start, check that:
• the dispensing head is not in a dispensing position.
• the plate is inserted into the instrument.
• the following liquids are available:
• washing liquid 1 (0.1 M NaOH solution)
• distilled water
• washing liquid 2 (0.1 M HCl)
1. Place the dispensing head into a waste vessel.
2. Place the aspirate tubing into washing liquid 1.
3. Flush the dispenser tubing. Select 1 cycle and click Flush. After the
flushing, the whole syringe is filled with the washing liquid.
4. Soak the syringe for 1 to 60 minutes. Select the soaking time (default
is 10 minutes), the cycles and click Soak.
The syringe is filled with the washing liquid for the defined time.
5. Place the aspirate tubing into distilled water.
6. Flush the dispenser syringe and tubing between 1 to 20 times. Select
the required 1 to 20 cycles and click Flush.
The syringe and tubing are flushed with distilled water into the waste
vessel.
7. Place the aspirate tubing into washing liquid 2.
8. Flush the dispenser tubing. Select 1 cycle and click Flush. After the
flushing, the whole syringe is filled with the washing liquid.
9. Soak the syringe for 1 to 60 minutes. Select the soaking time (default
is 10 minutes), the cycles and click Soak.
The syringe is filled with the washing liquid for the defined time.
10. Place the aspirate tubing into distilled water.
11. Flush the dispenser syringe and tubing between 1 to 30 times. Select
the required 1 to 30 cycles and click Flush.
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Executing a Session
Washing dispensers
79