Fluorometric Measurement - Thermo Scientific Varioskan User Manual

Fluorometric
measurement
Thermo Fisher Scientific
(see Measuring new pathlength correction K-factors" on
page 73).
For more information on pathlength correction and pathlength correction
factors, see Editing pathlength correction K-factors" on page 247 and
Pathlength correction" on page 72.
The Fluorometric Measurement step is used for measuring fluorescence
intensity (FI, RFU) at selected excitation and emission wavelengths.
Select Steps > Fluorometric or click the
The fluorometric measurement step includes the following parameters:
• Excitation wavelength [nm] - Select the excitation wavelength to be
used in the measurement. The wavelength range is 200 to 1,000 nm;
the default value is 485 nm.
• Emission wavelength [nm] - Select the emission wavelength to be used
in the measurement. The wavelength range is 270 to 840 nm; the default
value is 518 nm.
• Excitation bandwidth [nm] - Select the bandwidth of the excitation
wavelength to be used in the measurement. Bandwidth is selected using
an excitation slit that has 5 nm and 12 nm options. The default value
is 12 nm. The emission bandwidth is permanently set to 12 nm and
cannot be changed.
Note To prevent any stray light from the excitation light from
interfering with the emission light, the difference between the
Thermo Scientific SkanIt

Fluorometric measurement

button on the action panel.
® ®
Software for Varioskan User Manual
Step Parameters
99