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Blank subtraction
Thermo Fisher Scientific
Chapter 10
Data Processing Parameters
This chapter contains information describing all data processing (calculation)
parameters.
Blank subtraction is used for subtracting the mean blank value from all the
wells in the layout. Specific blanks are subtracted from their own samples
and no normal blank average is subtracted from samples with specific blanks.
Note When calculating the blank subtraction for dilutions series,
the mean value of all the specific blanks of the series is calculated.
The mean blank value is then subtracted from all the samples,
replicates, and blanks in the series.
Select Data Processing > Blank Subtraction, click the
Results action panel, or right-click the measurement in the Results tree.
•
Average - The average of all non-specific blanks in an assay is calculated
and subtracted from all the samples in the same assay (blanks, calibrators,
controls and unknowns). The blanks can be on the same plate or on
different plates, but they are all in the same assay and they are subtracted
from all the samples belonging to the same assay. See Example 10-1,
Average blank subtraction". Specific blanks are subtracted from their
own samples.
•
Plate blank - The average of all non-specific blanks available on each
plate in each assay is calculated separately. The average of the blanks
from each plate in each assay is then subtracted from the samples on
each specific plate in the same assay only. See Example 10-2, Plate
blank subtraction". Specific blanks are subtracted from their own
samples.
Thermo Scientific SkanIt
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Software for Varioskan
User Manual
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