Effective Dose; Figure 10-2 Effective Dose (Ed) Determination - Thermo Scientific Varioskan User Manual

Data Processing Parameters

Effective dose

Effective dose
164 Thermo Scientific SkanIt
The ED
50
of the sample has been lost. Typically, the followed activity is cell death or
cell survival or certain enzymatic activity. This value is used to demonstrate
the efficiency (such as toxicity) of the sample. In addition to ED
ED values are also counted, typically ED
concentration where 20% of the activity is lost, and similarly, ED
value where 80% has been lost. An example of the ED determination is
shown in Figure 10-2.
Figure 10-2. Effective dose (ED) determination
The curve values are defined according to the following equations:
where X is the sample selected as the ED
maximum signal).
When the ED value determination has been performed, the software also
automatically generates the graphics presenting the results. All results (curves
and ED values) are collected into one graph from the selected assay. The
calculated ED values are also shown in a list.
Select Data Processing > Effective Dose, click the
action panel, or right-click the measurement in the Results tree. Select the
Parameters tab to edit the calculation parameters.
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Software for Varioskan User Manual
value is a concentration value where 50% of the followed activity
and ED . The ED
20 80
(or the sample giving the
100
button on the Results
Thermo Fisher Scientific
, other
50
value is a
20
is the
80