Database - Thermo Scientific Varioskan User Manual

Thermo Fisher Scientific
The instrument reads photometrically the middle of the plate. If there
is no plate present in the instrument, you will receive an error message.
If the protocol has a Dispense step, the plate will always be checked,
regardless of the setting here.
• Optical response compensation - In fluorometry instrumentation, the
emission reading ability is not necessarily comparable between
wavelengths. This happens because the components of the optical system
do not have constant responses over the emission wavelength range.
Therefore this causes results which are dependent on the emission
wavelength even with the same excitation conditions. This uneven
emission response can cause difficulties in interpreting the spectral
results. For example, the emission peaks can shift even more than 10
nm.
The same applies also to the excitation response.
When selected (by default), the instrument compensates for the different
wavelength dependent response characteristics of the optical system in
both the emission and excitation. As a result, the RFU values between
different wavelengths are comparable with each other and the spectral
peaks correspond to the real light emitted from the sample.
If not selected, all the optical component wavelength dependent effects
influence the results.

Database

The Settings > Options... > Database tab is used to check and/or change
the working database used by SkanIt Software (see Appendix B: Database
maintenance").
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Thermo Scientific SkanIt Software for Varioskan
Settings
Options
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User Manual 227