Data Normalization - Thermo Scientific Varioskan User Manual

Data normalization

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into a single value. It can be used to calculate the statistics within each
sample in case of dilution series.
For example, a multipoint measurement with input data of eight
dilutions and two replicates for each dilution results in one value. The
average of the multipoint, replicate and dilution data is calculated and
the SD and CV% of the dilution values are calculated. The values are
multiplied by dilutions before calculating the average.
Note If the dilution factors are already calculated, they are not
calculated in subsequent basic statistics calculations.
The basic statistics calculates the average (Avg), the standard deviation (SD)
and the coefficient of variation (CV%) of the replicates.
The SD is calculated using the following formula of variance:
In the formula, x is the measured value from a single measurement point,
m is the mean of the measured replicate values, and n is the number of
replicates.
The average is displayed in both the table and list views and the SD and
CV% only in the list view.
To compare how the basic statistics and the user-defined equation calculate
the average, see the section called Comparison of the calculation of average
in User-Defined Equation and Basic Statistics".
Select Data Processing > Data Normalization, click the
Results action panel, or right-click the measurement in the Results tree.
• Calculation - Select either Ratio or Inhibition.
Thermo Scientific SkanIt
Data Processing Parameters
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Software for Varioskan User Manual
Data normalization
button on the
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