Thermo Scientific Varioskan User Manual page 84

Protocols
Editing a protocol
82 Thermo Scientific SkanIt
plate of the same size; for example, a 96-well plate can only be changed
into another 96-well plate.
Note If the protocol has multipoint measurements, changing the
plate template clears all the multipoint selections. You must confirm
the change first.
• Use default plate type - Tick the check box to use the default plate
type. The default is set in Settings > Plate Template (see Plate template
settings" on page 238).
• Well interval - Define the time that elapses between the starting times
of two consecutive wells. If selected, the value must be at least 10 ms
greater than the Settle delay value.
• Settle delay [ms] - Define the time delay after the plate has moved to
a new reading position before the instrument performs the following
reading. The range is 1 to 10,000 ms (10 seconds) and the default value
0 ms.
When liquid in the wells is exposed to acceleration or deceleration,
surface resonance waves occur in the wells. As the plate moves fast from
one well and stops at the next well prior to a measurement, the surface
waves start propagating in the liquid. The propagation continues for a
certain time depending on the liquid and the well size.
The surface waves may affect the results and thus it is necessary to ensure
that certain actions are taken to optimize the measurement. The surface
wave effect can be seen as noise in the signal in certain cases. There are
two methods to minimize surface wave effects when they occur:
1.
2.
• Execution order - Select the direction in which the steps are executed
on the plate. It also sets the direction in which the points inside a well
are measured in a multipoint measurement. The black dot designates
the starting point and the arrows show the execution order (Figure 6-1).
Use the up and down arrows to switch between the different options.
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Use detergent in the well, if possible.
Increase the Settle delay time and wait until the surface waves have
been sufficiently reduced. For example, in water-based samples the
surface waves may keep propagating up to:
• 5 seconds in a 6-well plate.
• 300 ms in a 96-well plate.
• < 20 ms in a 384-well plate.
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