Thermo Scientific Varioskan User Manual page 102

Step Parameters
Fluorometric measurement
100 Thermo Scientific SkanIt
excitation and emission wavelengths should be at least the sum of
the bandwidths used. If the 5 nm excitation and 12 nm emission
(permanent) bandwidths are used, the difference between excitation
and emission wavelengths should be at least 17 nm. With the 12 nm
excitation and emission bandwidths the difference should be at
least 24 nm.
• Dynamic range - Select the dynamic range to be used in the
measurement: AutoRange, Low, Medium Low, Medium High, or High.
The default selection is AutoRange. For more information, see Dynamic
range selections" on page 112.
• Measurement time [ms] - The measurement or integration time defines
the light collection time used in the measurement. The wells are
measured with a selected measurement time. For more information, see
Measurement times" on page 113.
• Lag time [hh:mm:ss.t] - Define the time before the measurement is
executed. Lag time is not available for kinetic loops.
• Step duration [hh:mm:ss] - Define the total time for the step. The
software waits after the instrument has performed the action until the
time is up before proceeding with the following step. Step duration is
not available for kinetic loops.
• Multipoint - Tick the check box to select which points in a well are
measured. If the multipoint measurement is not selected, only the center
of the well is measured. Multipoint is only available for 6 to 48-well
plates. For more information, see Using multipoint in a measurement"
on page 114.
Note If there are not any points selected in the multipoint
measurement, the protocol cannot be run.
• Optics - Fluorescence can be measured from one position:
• Top - Fluorescence is measured at the measurement position above
the plate.
• Bottom - This option is available with the Varioskan Flash only.
• Multiple measurements - Tick the check box to enter multiple
wavelength pair values. All wavelengths are measured one after the other
from the same well before moving to the next well. For more
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