Thermo Scientific Vanquish VH-D10 Operating Manual
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Thermo Scientific Vanquish VH-D10 Operating Manual

Diode array detectors
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Vanquish
Diode Array Detectors
VH-D10
Operating Manual
4820.8301-EN Revision 3.0
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January 2023
*4820.8301-EN-3.0*

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  • Page 1 Vanquish Diode Array Detectors VH-D10 Operating Manual 4820.8301-EN Revision 3.0 • January 2023 *4820.8301-EN-3.0*...
  • Page 2 Copyright © 2023 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual The hardware descriptions in this manual revision refer to devices: VH-D10-A. Trademarks Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated. Microsoft and Windows are trademarks of Microsoft Corporation. Torx is a trademark of Acument Intellectual Properties, LLC.
  • Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermofisher.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
  • Page 4 Contacting Us Page 4 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 5: Table Of Contents

    Contents Contents 1 Using this Manual...............  11 About this Manual .................... 12 Conventions ...................... 13 1.2.1 Conventions for Safety Messages ............ 13 1.2.2 Special Notices .................. 13 1.2.3 Typographical Conventions .............. 14 Reference Documentation...................  15 2 Safety .................. 17 Safety Symbols and Signal Words ................  18 2.1.1 Safety Symbols and Signal Words in this Manual........
  • Page 6 Contents Leak Detection .....................  39 Operation...................... 40 4 Unpacking ..................  41 Unpacking ...................... 42 Scope of Delivery .................... 44 5 Installation .................  45 Safety Guidelines for Installation .................  46 Installing the Device.....................  47 Site Requirements.................... 49 5.3.1 Power Considerations ................ 49 5.3.2 Power Cord....................
  • Page 7 Contents 6 Operation...................  83 Introduction to this Chapter ................ 84 Safety Guidelines for Operation................ 85 Control Elements .................... 86 6.3.1 Keypad......................  86 6.3.2 Status Indicators.................. 87 Power On/Off Control.................. 89 Preparing the Device for Operation.............. 90 Turning On the UV Lamp.................. 92 Guidelines for Use of Flow Cells................
  • Page 8 Contents Flow Cell...................... 126 7.7.1 Preparing the Flow Cell for Storage ............ 126 7.7.2 Removing the Flow Cell................ 129 7.7.3 Installing the Flow Cell ................ 130 7.7.4 Back-Flushing the Flow Cell.............. 134 Diagnostic Cell.................... 142 7.8.1 Installing the Diagnostic Cell .............. 142 7.8.2 Removing the Diagnostic Cell..............
  • Page 9 Contents 10.3 Optional Accessories.................. 180 10.4 Consumables and Replacement Parts.............. 181 11 Appendix..................  183 11.1 Compliance Information .................. 184 11.1.1 Declarations of Conformity .............. 184 11.1.2 WEEE Compliance ..................  185 11.1.3 FCC Compliance.................. 185 11.1.4 NIST Compliance .................. 185 11.1.5 Manual Release History................ 186 11.2 UV Cutoff Wavelengths of Solvents ..............
  • Page 10 Contents Page 10 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 11: Using This Manual

    • Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Diode Array Detectors (VH-D10) Page 11 Operating Manual...

  • Page 12: About This Manual

    • Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ device and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. This manual also contains safety messages, precautionary statements, and special notices.

  • Page 13: Conventions

    • Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Conventions for Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.

  • Page 14: Typographical Conventions

    • Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output The following appears in bold type: • Input that you enter by the keyboard or that you select with the mouse •...

  • Page 15: Reference Documentation

    • Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system • Vanquish System Operating Manual •...
  • Page 16 • Using this Manual Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Page 16 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 17: Safety

    • Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device. Diode Array Detectors (VH-D10) Page 17 Operating Manual...

  • Page 18: Safety Symbols And Signal Words

    • Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in this Manual This manual contains safety messages to prevent injury of the persons using the device. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.

  • Page 19: Safety Symbols On The Device

    • Safety 2.1.3 Safety Symbols on the Device The table lists the safety symbols that appear on the device or on labels affixed to the device. Follow the safety notices in this manual to prevent the risk of operator injury or damage to the device. Symbol Description Indicates a potential hazard.

  • Page 20: Intended Use

    • Safety Intended Use The device is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The device is for use by qualified personnel and in laboratory environment only.

  • Page 21: Safety Precautions

    • Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the device. If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired.

  • Page 22: Qualification Of The Personnel

    • Safety 2.3.2 Qualification of the Personnel Observe the information below on the proper qualification of the personnel installing and/or operating the device. Installation Only skilled personnel are permitted to install the device and to establish the electrical connections according to the appropriate regulations.

  • Page 23: Electrical Safety Precautions

    • Safety Protective Clothing To protect you from chemical splashes, harmful liquids, or other contamination, put on appropriate protective clothing, such as a lab coat. Protective Eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles.

  • Page 24: General Residual Hazards

    • Safety 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the device: WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...
  • Page 25 • Safety WARNING—Self-Ignition of Solvents Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. WARNING—Hazardous Vapors Mobile phases and samples might contain volatile or flammable solvents.

  • Page 26: In Case Of Emergency

    • Safety CAUTION—Sparking due to Electrostatic Discharge Liquid flowing through capillaries can generate static electricity. This effect is particularly present with insulating capillaries and non- conductive solvents (for example, pure acetonitrile). Discharge of electrostatic energy might lead to sparking, which could constitute a fire hazard.

  • Page 27: Solvent And Additive Information

    • Safety Solvent and Additive Information 2.4.1 General Compatibility To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. •...

  • Page 28: Allowed Concentrations

    • Safety 2.4.3 Allowed Concentrations Allowed concentrations (standard system configuration): System Chloride Buffer Remarks (Standard Configuration) Vanquish Core 0.1 mol/L 1 mol/L or High chloride concentration: The or less less application time should be as short as possible. Flush the Vanquish Horizon 1 mol/L or system thoroughly after these...

  • Page 29: Compliance Information

    • Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.
  • Page 30 • Safety Page 30 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 31: Device Overview

    • Device Overview 3 Device Overview This chapter introduces you to the device and the main components. Diode Array Detectors (VH-D10) Page 31 Operating Manual...

  • Page 32: Detector Features

    • Device Overview Detector Features The device comprises the following main features: • A deuterium lamp for ultraviolet (UV) and visible (VIS) wavelength detection as the light source of the device The deuterium lamp provides light for the complete wavelength detection range from 190 nm to 680 nm.

  • Page 33: Operating Principle

    • Device Overview Operating Principle The device is designed for ultraviolet (UV) and visible (VIS) absorption spectroscopy in combination with HPLC or UHPLC separations. After sufficient separation from other sample compounds, the analysis of the target compound follows the Beer-Lambert law. This means that the response of the device is proportional to the concentration of the analyte.
  • Page 34 • Device Overview The light passes through the sample flow path in the light pipe of the flow cell. After exiting the flow cell through the exit fiber, the light hits the spectrograph mirror. The spectrograph mirror (5) focuses the light to the adjustable entrance slit (6) of the spectrograph.

  • Page 35: Interior Components

    • Device Overview Interior Components The user-accessible components of the device are located directly behind the front doors: Figure 2: Interior view (here with flow cell installed) Description Cooling air intake Capillary guide slit below the cooling air intake To hold the capillary if the column compartment is located to the left of the device.

  • Page 36: Flow Cell

    • Device Overview Flow Cell The detector design allows easy access to the flow cell on the interior front. Figure 3: Flow cell (example) Description Outlet (OUT) (closed with plug during storage and transport) Inlet (IN) (closed with plug during storage and transport) Flow cell label Rotating locks (unlocked position) Flow Cell Label...
  • Page 37 • Device Overview The flow cells are optimized for highest possible light transmission and transmission stability for the complete wavelength range of the device from 190 nm to 680 nm. The following flow cells are available for the device: • LightPipe flow cell, standard, path length 10 mm •...

  • Page 38: Lamp

    • Device Overview Lamp As light source for ultraviolet (UV) and visible (VIS) wavelength detection, the deuterium lamp provides light for the complete wavelength detection range from 190 nm to 680 nm. The lamp is equipped with an identification (ID) chip. The ID chip stores information about the lamp, including the number of lamp ignitions and the operating time of the lamp, thus, providing an overview of the lamp status.

  • Page 39: Leak Detection

    • Device Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the device monitors the device for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.

  • Page 40: Operation

    Operating Manual. Details on control and operation of the device are available in the Chromeleon Help. TIP The device can be operated also with other data systems, such as Thermo Scientific Xcalibur . In this case, installation of additional software is required in addition to the data system software. For details, contact the Thermo Fisher Scientific sales organization.

  • Page 41: Unpacking

    • Unpacking 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery. Diode Array Detectors (VH-D10) Page 41 Operating Manual...

  • Page 42: Unpacking

    • Unpacking Unpacking Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the device for any signs of mechanical damage that might have occurred during shipment. If you suspect that the device may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
  • Page 43 • Unpacking 3. Remove the device from the shipping container: Grasp the device by the carrying handles. Slowly and carefully, lift the device out of the shipping container. Figure 5: Carrying handles on the device Component Carrying handles Attachment screw (one on each carrying handle) 4.

  • Page 44: Scope Of Delivery

    • Unpacking Scope of Delivery The following items are included in the delivery: • Detector • Ship Kit • Operating manual (downloadable from customer manual web site) • Power cord For information on contents of the ship kit or reordering parts, see Accessories, Consumables and Replacement Parts (} page 177).

  • Page 45: Installation

    • Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the device in the Vanquish system and in the chromatography software. Diode Array Detectors (VH-D10) Page 45 Operating Manual...

  • Page 46: Safety Guidelines For Installation

    • Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...

  • Page 47: Installing The Device

    • Installation Installing the Device A Thermo Fisher Scientific service engineer installs and sets up the Vanquish system, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified. The engineer also demonstrates the basic operation and main features.
  • Page 48 • Installation 6. Recommended: Perform Instrument Installation Qualification. In the Chromeleon software, a wizard is available to guide you through the qualification process. On the Chromeleon 7 Console: Click Tools > Instrument Qualification > Installation Qualification. Follow the instructions in the Instruments Installation Qualification Operating Instructions.

  • Page 49: Site Requirements

    • Installation Site Requirements The operating environment is important to ensure optimal performance of the device. This section provides important requirements for the installation site. Note the following: • Operate the device only under appropriate laboratory conditions. • The device is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.

  • Page 50: Condensation

    • Installation WARNING—Electric Shock or Damage to the Device • Never use a power cord other than the power cords provided by Thermo Fisher Scientific for the device. • Only use a power cord that is designed for the country in which you use the device.

  • Page 51: Accessing The Interior Components

    • Installation Accessing the Interior Components To access the interior components in the device, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the device are located directly behind the doors. Figure 7: Opening the front doors Diode Array Detectors (VH-D10) Page 51...

  • Page 52: Setting Up The Hardware

    • Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the device connectors and cables. 5.5.1 System Arrangement The device is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.

  • Page 53: Connecting The Device

    • Installation 5.5.2 Connecting the Device Device Connectors The following connectors are provided on the device: Figure 9: Electrical connectors on the right side of the device Description Rating plate, indicating the serial number, part number, module name, revision number (if any), and the line and fuse rating. Main power switch (on/off control) Fuse holder Power-inlet connector...

  • Page 54: Connecting The Power Cord

    • Installation TIP Thermo Fisher Scientific recommends using the USB ports only as described above. If the USB ports are used for any other purpose, Thermo Fisher Scientific cannot ensure proper functionality. Follow these steps NOTICE • Never use defective communication cables. If you suspect that a cable is defective, replace the cable.

  • Page 55: Installing The Flow Cell

    • Installation Installing the Flow Cell This section describes the installation of the flow cell upon initial installation of the device. For instructions on exchanging a flow cell or installing a flow cell after storage, see Flow Cell (} page 126). NOTICE—Sensitive Flow Cells Flow cells are highly sensitive to damage.
  • Page 56 • Installation Parts required Flow cell Figure 10: Front side of the flow cell Description Outlet (OUT) (closed with plug during storage and transport) Inlet (IN) (closed with plug during storage and transport) Flow cell label Rotating locks (unlocked position) Preparations 1.
  • Page 57 • Installation Figure 11: Flow cell opening on the device Description Contact pad for flow cell identification chip Optical ports in the device 3. Unpack the cell. Follow these steps 1. Remove the shipping locks carefully on the left and right rear side of the flow cell.
  • Page 58 • Installation 2. Check the position of the rotating locks on the front of the flow cell. If the rotating locks are not in a horizontal position, turn them counterclockwise to a horizontal position. To insert the flow cell, the rotating locks must always be in a horizontal position and thus opened.

  • Page 59: Setting Up The Flow Connections

    • Installation Setting Up the Flow Connections 5.7.1 General Information and Guidelines When setting up flow connections, follow these rules and recommendations: Flow connections can be filled with hazardous substances. Observe the warning messages and precautionary statements presented in Safety Precautions (} page 21).

  • Page 60: Guiding Capillaries And Tubing Through The System

    • Installation • If using multi-detector configurations, configurations that include a diverter valve, and hyphenated techniques like LC-MS or fractionation after a LightPipe flow cell, install an overpressure relief valve. The valve is intended to limit pressure in an error case. It is not intended to limit overpressure events as a consequence of the system configuration and/or instrument method not observing the flow cell pressure limit.
  • Page 61 • Installation Each guide can hold up to three tubes or lines. In each module, push the tube (or line) into the appropriate guide. Figure 15: Tubing chase with tubing guides (left: view from inside, right: view from top) Use for Solvent tubing (up to three solvent lines) Solvent tubing (up to three solvent lines) Wash liquid tubing (seal wash, autosampler needle wash)

  • Page 62: Installing The Partition Panel Plugs

    • Installation Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules. Route flow connections from one module to the next module in the Vanquish system through the appropriate guide hole or capillary clip when instructed to do so in the manual.
  • Page 63 • Installation 3. To secure the capillary, push the capillary into the slit of the plug. Figure 19: Capillary installed in the plug with slit Installing the rotating plug 1. On the detector partition panel, push the rotating plug in the required recess of the partition panel (if not present yet).

  • Page 64: Connecting Fittings, Capillaries, And Tubing

    • Installation 5.7.4 Connecting Fittings, Capillaries, and Tubing This section provides information about how to connect and handle capillaries, fittings, and tubing. 5.7.4.1 General Guidelines When connecting capillaries and tubing, follow these general recommendations: • Use only the capillaries and tubing (for example, solvent lines or waste tubing) that are shipped with the product or additional or spare capillaries and tubing as recommended by Thermo Fisher Scientific.
  • Page 65 • Installation Figure 21: Viper fitting with knurl Description Knurl Capillary Slot 1. Insert the Viper capillary into the connection port. 2. Tighten the connection by the knurl. TIP Note the slot in the knurl. You can easily remove the knurl from the capillaries through this slot if space is limited.

  • Page 66: Flow Connections To The Flow Cell

    • Installation 5.7.5 Flow Connections to the Flow Cell Connect the inlet capillary and waste line to the flow cell when the flow cell is installed to the device. NOTICE Flow cells are highly sensitive to contamination, clogging and high backpressures.
  • Page 67 • Installation Parts required • Inlet capillary • Detector waste line For instructions on connecting the waste line, follow the steps in Connecting the Detector Waste Line (} page 69). • Plugs for the partition panel from the detector ship kit (if not pre- installed), depending on the capillary to be installed: For capillaries with small outer diameter, such as uninsulated ¨...
  • Page 68 • Installation Follow these steps 1. Connect the inlet of the flow cell: Connect the capillary from the column to the flow cell. See Connecting the Inlet Capillary (} page 68). 2. Connect the outlet of the flow cell: If the detector is the last module in the system flow path, ¨...
  • Page 69 • Installation 2. If the column compartment is located to the right of the detector Route the capillary through the bottom recess in the partition panel. Make sure that you use the suitable partition panel plug for the capillary. TIP For capillaries with small outer diameter, the plug with slit secures the capillary properly.

  • Page 70: Guiding Liquid Leaks To Waste

    • Installation 5. If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line and that the cut is not crimped. 6. Check the waste line over the entire flow path: Make sure that no bending (kink), pinching or squeezing of the waste line is present at any point in the flow path.

  • Page 71: Determining The Pressure Inside The Flow Cell

    • Installation Determining the Pressure inside the Flow Cell All modules, capillaries and waste lines downstream of the flow cell contribute to the pressure inside the flow cell. If you attach additional modules such as detectors, fraction collectors or a mass spectrometer in the flow path after the flow cell, make sure that the pressure within the flow cell does not exceed its pressure specification.
  • Page 72 • Installation General Outline of the Procedure 1. Measure the backpressure of the waste line (see Measuring the Backpressure of the Waste Line (} page 73)). Flow path: Pump – waste line 2. Measure the backpressure of the Vanquish system including the column, detector inlet capillary and waste line (without installed flow cell and additional module) (see Measuring the Vanquish...

  • Page 73: Measuring The Backpressure Of The Waste Line

    • Installation 5.8.1 Measuring the Backpressure of the Waste Line This procedure describes how to measure the backpressure of the waste line (p0). Figure 25: Measuring the backpressure of the waste line Description Description Detector Column Flow cell Waste container Outlet port of the flow cell Second detector Inlet port of the flow cell Waste line...

  • Page 74: Additional Module)

    • Installation 7. When the system pressure has stabilized, read the system pressure and write down the value for p0. p0: Backpressure caused by the waste line 8. Remove the waste line from the pump/static mixer outlet port. 9. Connect the autosampler inlet capillary to the pump/static mixer outlet port.
  • Page 75 • Installation 1. If applicable, disconnect the detector inlet capillary from the flow cell IN port. 2. If applicable, disconnect the waste line from the flow cell OUT port. 3. Connect the free end of the detector inlet capillary to the union connector.

  • Page 76: Determine The Backpressure At The Flow Cell Outlet Caused By The Connected Additional Module (Without Flow Cell)

    • Installation 5.8.3 Determine the Backpressure at the Flow Cell Outlet Caused by the Connected Additional Module (Without Flow Cell) This procedure first describes how to measure the system backpressure with the additional module (p2), but with the flow cell replaced by the union connector.
  • Page 77 • Installation 4. Connect the inlet port of the additional module to the free end of the union connector with the transfer capillary which will be used to connect the additional module in the application setup. 5. Start the pump flow at the maximum flow rate of your application. 6.

  • Page 78: Determining The Backpressure Of The Flow Cell

    • Installation 5.8.4 Determining the Backpressure of the Flow Cell This procedure first describes how to measure the system backpressure with flow cell but without additional module (p4). The backpressure of the flow cell (p5) is then calculated by subtracting the Vanquish system backpressure without flow cell and additional module (p1).

  • Page 79: Calculating The Pressure At The Inlet Port Of The Flow Cell

    • Installation 6. Calculate the difference between the two measured pressure values: p5 = p4 – p1. p5: Backpressure caused by the flow cell p4: System backpressure with flow cell but without additional module p1: Vanquish system backpressure (without flow cell and additional module) 5.8.5 Calculating the Pressure at the Inlet Port of the Flow Cell...
  • Page 80 • Installation 1. Calculate p6 = p5 + p3. p6: Pressure at the inlet port of the flow cell p5: Backpressure caused by the flow cell p3: Backpressure the connected additional module produces at the flow cell outlet 2. Compare p6 with the pressure limit of the flow cell. Situation Steps p6 is at least 5 bar below...

  • Page 81: Turning On The Device

    • Installation Turning On the Device Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...

  • Page 82: Setting Up The Device In The Software

    • Installation 5.10 Setting Up the Device in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.

  • Page 83: Operation

    • Operation 6 Operation This chapter describes the elements for device control, provides information for routine operation and for shutdown. Diode Array Detectors (VH-D10) Page 83 Operating Manual...

  • Page 84: Introduction To This Chapter

    • Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the device has already been completed. If this is not the case, see the instructions in Installation (} page 45). For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.

  • Page 85: Safety Guidelines For Operation

    • Operation Safety Guidelines for Operation When operating the device, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Hot Surfaces Surfaces inside the device may become hot during operation. Touching hot parts might cause burns.

  • Page 86: Control Elements

    • Operation Control Elements The device is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the device: • Keypad The keypad buttons allow you to perform certain functions directly from the device.

  • Page 87: Status Indicators

    • Operation MUTE ALARM Beeping alerts you when the device detects a problem, for example, leakage. To turn off the beep for the current alarm, press this button. Eliminate the source for the alarm within 10 minutes. Otherwise, beeping starts again. If the device detects a different problem, beeping alerts you again immediately.
  • Page 88 • Operation LED Bar Description Yellow The device is connected in the Chromeleon software, but the device is not equilibrated. The UV lamp is turned off, or no flow cell (or diagnostic cell) is installed. Green, flashing The device is equilibrating. The UV lamp is igniting. Green The device is connected and equilibrated, but no data acquisition is running.

  • Page 89: Power On/Off Control

    • Operation Power On/Off Control The power switch on the device is the main switch for power on/off control. The main power switch is turned on during initial installation of the device. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.

  • Page 90: Preparing The Device For Operation

    • Operation Preparing the Device for Operation This section gives information on any additional steps that are required to prepare the device for operation and sample analysis. Before Operating the Device for the First Time Prepare the device for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the device for the first time:...
  • Page 91 • Operation System Equilibration System equilibration should include the following operations: • Purging the pump (all channels, including those not used for the application) • Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run •...

  • Page 92: Turning On The Uv Lamp

    • Operation Turning On the UV Lamp Prerequisites The lamp house cover must be installed. TIP If you remove the lamp house cover while the lamp is turned on, the lamp is turned off automatically. Follow these steps 1. Press the UV button on the keypad of the detector. - or –...

  • Page 93: Guidelines For Use Of Flow Cells

    • Operation Guidelines for Use of Flow Cells NOTICE—Highly sensitive flow cells Flow cells are highly sensitive to damage. • Handle flow cells with care. • Observe the following guidelines for use of the flow cell. Operating conditions • Observe the specified maximum temperature limit and pressure limit for the flow cell.
  • Page 94 • Operation Devices in the Flow Path After the Flow Cell NOTICE If using multi-detector configurations, configurations that include a diverter valve, and hyphenated techniques like LC-MS or fractionation after a LightPipe flow cell, install an overpressure relief valve. The valve is intended to limit pressure in an error case.
  • Page 95 • Operation • During long analysis breaks, take appropriate measures to protect the flow cell: Turn off the lamps to protect the flow cell from the light. ¨ Otherwise, the radiation from the lamps may damage the flow cell. If the pump flow is stopped for a longer time, fill the flow cell ¨...

  • Page 96: Important Operating Parameters

    • Operation Important Operating Parameters The commands and parameters described in the table should be considered for simple routine operation of the device. You can usually access these parameters from the Chromeleon user interface. If a parameter listed below is not available in the Chromeleon software, consider updating the firmware and Chromeleon version.
  • Page 97 • Operation Parameters Description Wavelength Set a wavelength for each UV_VIS_x signal channel individually, in the range of 190 nm to 680 nm for up to 10 signal channels. The wavelength defines the value at which the device measures the absorbance of the analyte(s) in the sample. Observe the following: •...
  • Page 98 • Operation Parameters Description Peak width The peak width is a supporting parameter for the data collection rate setting and the response time. As a standard, the Peak Width setting is defined when the data collection rate is set. If required, you can set a specific value for the peak width.
  • Page 99 • Operation Parameters Description Autozero Perform an automatic null balancing. The current device signal is interpreted as 0. Therefore, no absorbing sample should be in the flow cell when Autozero is performed. TIP When you start the data acquisition or perform an autozero, the shutter in the light path before the flow cell is opened automatically.

  • Page 100: Optimizing The Performance Of The Device

    • Operation Optimizing the Performance of the Device This section provides information for best performance of the device and gives hints on what you can do to optimize the performance further. 6.9.1 Guidelines for Optimum Performance Consider the following guidelines for optimization of the device performance: •...

  • Page 101: Overview Of Optimization Parameters

    • Operation 6.9.2 Overview of Optimization Parameters The following table serves as an overview of parameters that influence the spectral averaging, and, in case of the data collection rate and the response time, the time averaging during data acquisition. Parameter Affects Wavelength Sensitivity, linearity...

  • Page 102: Selecting The Response Time And Peak Width

    • Operation 6.9.4 Selecting the Response Time and Peak Width As a standard, when setting the data collection rate, Chromeleon automatically sets the optimum response time and peak width. As an advanced option, response time and peak width can be set individually. Disabling the link between the data collection parameters and setting these parameters individually may lead to increased noise or increased peak widths.
  • Page 103 • Operation Note the following: • The averaging always takes place symmetrically to the selected wavelength. • When performing a detection near the edge of the spectral range If the bandwidth setting results in an averaging asymmetrically to the selected wavelength, the device will correct the setting. Example: If you perform a detection at a wavelength of 195 nm with a bandwidth of 20 nm, the photodiode bunching is set to 190 to 200 nm, i.e.

  • Page 104: Selecting The Reference Wavelength And Bandwidth

    • Operation 6.9.6 Selecting the Reference Wavelength and Bandwidth Some disturbances on the measured signal from the sample can be corrected by a measured reference signal, which is defined by the parameters reference wavelength and reference bandwidth. Reference Wavelength Interfering substances can absorb in addition to the absorption of the analyte in the sample.
  • Page 105 • Operation Reference Bandwidth If the reference wavelength is used, the reference bandwidth determines how many photodiode signals are averaged for the reference measurement. You can set the reference bandwidth separately for each signal channel and the 3D field. Select a reference bandwidth that is as broad as possible, such as 30 –  100 nm.

  • Page 106: Shutting Down The Device

    • Operation 6.10 Shutting Down the Device If the device will not be operated for some time, follow the instructions in this section to shut down the device. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.

  • Page 107: Long-Term Shutdown

    • Operation • For flow cells, note the following: If the pump flow is interrupted and the lamp is turned on, ¨ protect the flow cell from the light of the lamp: Close the shutter in the light path before the flow cell, or turn off the lamp. If the pump flow is stopped for a longer time, or if the flow cell is ¨...
  • Page 108 • Operation Situation after If no additive is used If an additive is used Shutdown Device and flow cell Flush the system with Flush the system first with shall be transported isopropanol. several volumes of methanol or shipped after and water (50:50) (for shutdown example, 1.0 mL/min for 10 minutes with the standard...

  • Page 109: Restart After Long-Term Shutdown

    • Operation 9. The step depends as follows: Situation Steps Device and all other system Turn off the system with the system power button on modules remain in the the system base. system stack and are to be turned off Device shall be transported If one of the modules shall be removed from the or shipped after shutdown...
  • Page 110 • Operation Page 110 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 111: Maintenance And Service

    • Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Diode Array Detectors (VH-D10) Page 111 Operating Manual...

  • Page 112: Introduction To Maintenance And Service

    • Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).

  • Page 113: Safety Guidelines For Maintenance And Service

    • Maintenance and Service Safety Guidelines for Maintenance and Service 7.2.1 General When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). WARNING—High Voltage High voltages are present inside the device that could cause an electric shock.
  • Page 114 • Maintenance and Service CAUTION—Spraying Solvent Solvents can spray when under high pressure. • Stop the pump flow prior to opening the flow path. • Wait until the system pressure is down to zero. • When opening the flow path, wear appropriate protective equipment.

  • Page 115: Flow Cells

    • Maintenance and Service 7.2.2 Flow Cells NOTICE—Highly sensitive flow cells Improper use or handling can lead to increased noise, increased drift, increased refractive index sensitivity, clogging, leaks on the flow cell, or even destruction of the flow cell. • Handle flow cells always with care and use them only and strictly within their specifications of up to 6 MPa and 50 °C.

  • Page 116: General Rules For Maintenance And Service

    • Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow the rules and recommendations below. General Rules • Before starting maintenance or service procedures, shut down the device when instructed to do so. •...

  • Page 117: Routine And Preventive Maintenance

    • Maintenance and Service Routine and Preventive Maintenance Optimum device performance, maximum uptime of the device, and accurate results can be obtained only if the device is in good condition and properly maintained. 7.4.1 Maintenance Plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.
  • Page 118 • Maintenance and Service Decontamination Decontamination is required, for example, when leakage or spillage has occurred, or before service or transport of the device. Use a suitable cleaning detergent or disinfectant to ensure that the treatment renders the device safe to handle. Parts required •...

  • Page 119: Predictive Performance

    • Maintenance and Service Preparations 1. Turn off the power to the device and disconnect the power cord from the power source. Follow these steps 1. Wipe the surfaces clean with a clean, dry, soft, lint-free cloth or wipe. If necessary, slightly dampen the cloth or wipe with a solution of lukewarm water and a suitable cleaning detergent.

  • Page 120: Performing A Wavelength Validation And Wavelength Calibration

    • Maintenance and Service Performing a Wavelength Validation and Wavelength Calibration A holmium-oxide glass filter is used to validate the wavelength accuracy. The filter can be moved into the light path between the lamp and the flow cell for the wavelength validation. The detector determines the absorption maxima of the filter and compares them to the nominal holmium oxide values that are stored in the detector firmware.
  • Page 121 • Maintenance and Service To perform a wavelength validation 1. Turn on the flow at the flow rate that will be used for the analysis. 2. Ensure that the shutter is open. 3. In the chromatography data system, in the Commands window, execute the WavelengthValidation command.

  • Page 122: Replacing The Lamp

    • Maintenance and Service Replacing the Lamp When • The lamp is defective • After a defined amount of operating hours of the lamp as a preventive measure • The lamp intensity is too low for demanding applications • The lamp intensity fluctuates causing artifacts in the baseline Parts required •...
  • Page 123 • Maintenance and Service Follow these steps Figure 32: UV lamp Description UV lamp locating pin Screw bores for UV lamp attachment UV lamp connector 1. Open the doors. 2. Turn the screws on the lamp house cover counterclockwise until the lamp house cover is loose.
  • Page 124 • Maintenance and Service 3. Remove the lamp house cover. CAUTION—Hot surface The lamp may become hot. Touching a hot lamp might cause burns. • Touch the lamp briefly and carefully to find out if it is hot before you remove the lamp.
  • Page 125 • Maintenance and Service 11. Mind the routing of the lamp cables. Position the lamp cables as indicated in the figure above to prevent the cable from being pinched under the lamp house cover. 12. Reinstall the lamp house cover and fasten the screws on the lamp house cover.
  • Page 126 • Maintenance and Service Flow Cell This section describes how to flush and exchange flow cells. To store or ship the flow cell, follow these steps: 1. Flush the flow cell (see Preparing the Flow Cell for Storage (} page 126)). 2. Remove the flow cell from the device and place it in its packaging (see Removing the Flow Cell (} page 129)).
  • Page 127 • Maintenance and Service 3. Make sure that residual sample components, impurities from the column, aggressive solvents or eluates with salt contents are completely flushed out of the flow cell. 4. If the UV lamp is turned on Close the shutter in the light path before the flow cell. ‑or‑...
  • Page 128 • Maintenance and Service 7.7.1.1 Performing a Manual Injection A sample can also be injected manually using the flushing and injection kit. Parts required Flushing and injection kit for flow cells, including a syringe and a Viper adapter NOTICE Particulate matter from the eluate can deposit in the flow cell and clog •...
  • Page 129 • Maintenance and Service 7.7.2 Removing the Flow Cell When • Storing or transporting the flow cell in its packaging • Exchanging the flow cell • Leakage occurred on the flow cell Parts required • If the flow cell is to be stored Packaging of the flow cell, the shipping locks and the plugs •...
  • Page 130 • Maintenance and Service 6. If a flow cell or diagnostic cell is to be installed: For instructions on installing a flow cell, see Installing the Flow Cell (} page 130) and on installing a diagnostic cell, see Installing the Diagnostic Cell (} page 142).
  • Page 131 • Maintenance and Service • The optical ports of the flow cell are sensitive to contamination and scratches. Do not touch the optical ports of the flow cell or immerse them. To avoid damage to the optical ports of the flow cell, be careful when inserting the flow cell into the flow cell opening of the detector.
  • Page 132 • Maintenance and Service Follow these steps 1. Remove the shipping locks carefully on the left and right rear side of the flow cell. TIP Store the shipping locks in the cell packaging to have them easily available when storing or shipping the cell. Figure 35: Rear side of the flow cell Description Identification chip...
  • Page 133 • Maintenance and Service 3. Carefully insert the flow cell into the flow cell opening in the device. The flow cell must sit completely in the opening. Figure 36: Inserting the flow cell with opened rotating locks 4. Turn the rotating locks simultaneously clockwise until they are in a vertical position.

  • Page 134: Back-Flushing The Flow Cell

    • Maintenance and Service 7.7.4 Back-Flushing the Flow Cell If a flow cell shows increased backpressure or even blockage, back- flushing the flow cell at a high flow rate can help to regain the original performance of the flow cell. This can also be performed as a remedy if the light transmittance of the flow cell is reduced due to contamination of the light pipe, causing increased noise and instable baseline.
  • Page 135 • Maintenance and Service Parts required • Back-flush kit for flow cells Figure 38: Back-flush kit Description Back-flush capillary Inlet line for connection to the pump In-line filter Overpressure line to the waste T piece Flow cell capillary for connection to the flow cell Back-flush waste line Back-flush plug •...
  • Page 136 • Maintenance and Service Preparations The numbers in parentheses in the procedures below refer to figure Back-flush kit (} page 135) above. 1. Remove the flow cell from the detector (see Removing the Flow Cell (} page 129)). 2. Make sure that the solvent in the flow cell is miscible with the back- flushing agent.
  • Page 137 • Maintenance and Service 3. Disconnect the flow cell capillary from the back-flush capillary (no. 1e) and screw the back-flush plug (no. 3) into to the T piece (no. 1d). The image below shows the finalized setup for determining the pump flow.
  • Page 138 • Maintenance and Service Flushing the T piece 1. Remove the back-flush plug (no. 3) from the T piece (no. 1d). 2. Connect the back-flush waste line (no. 2) to the back-flush capillary (no. 1) and route the free end of the tubing to waste. The image below shows the finalized setup for flushing the T piece.
  • Page 139 • Maintenance and Service 5. Connect the flow cell capillary of the back-flush kit (no. 1e) to the flow cell outlet. The image below shows the finalized setup for back-flushing the flow cell. Figure 41: Setup for back-flushing (here: without overpressure relief valve) Description To the outlet of the pump...
  • Page 140 • Maintenance and Service Forward flushing 1. Switch the capillary connections on the flow cell: Connect the flow cell capillary of the back-flush capillary (no. 1e) to the flow cell inlet and the back-flush waste line (no. 2) to the flow cell outlet. The image below shows the finalized setup for forward flushing the flow cell.
  • Page 141 • Maintenance and Service 4. Monitor the operating pressure: Situation Reason Steps If the pressure The clogging was not Thermo Fisher Scientific remains high at a removed. recommends performing value similar to the another backward and value that you wrote forward flushing cycle: down 1.

  • Page 142: Diagnostic Cell

    • Maintenance and Service Diagnostic Cell When problems with the baseline noise, baseline drift or during wavelength validation occur, install the diagnostic cell and use it to identify the cause: • If the problems disappear with the diagnostic cell The problems are caused by the flow cell that was previously installed or the flow path or components in the flow path before the device.

  • Page 143: Removing The Diagnostic Cell

    • Maintenance and Service Follow these steps 1. Remove the shipping locks carefully on the left and right rear side of the diagnostic cell. TIP Store the shipping locks in the cell packaging to have them easily available when storing or shipping the cell. 2.
  • Page 144 • Maintenance and Service 4. Proceed as required: Situation Steps If a flow cell is to be Install the flow cell to the flow cell opening on the installed afterward device (see Installing the Flow Cell (} page 130)). If no flow cell is to be Install the cover to the flow cell opening on the device: installed afterward 1.

  • Page 145: Replacing The Waste Line

    • Maintenance and Service Replacing the Waste Line NOTICE Backpressures that exceed the specified maximum pressure limit of the flow cell can destroy the flow cell. Observe the following: • Use only the waste line connection that is provided for your detector.
  • Page 146 • Maintenance and Service 3. Remove the waste line from the top recess of the partition panel: If the waste line is installed in a rotating plug ¨ Turn the plug in the top recess to the front and remove the waste line from it.

  • Page 147: Replacing The Main Power Fuses

    • Maintenance and Service 7.10 Replacing the Main Power Fuses When Blown fuses Parts required Fuses (2 fuses, 3.15 AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock High voltages are present inside the device that could cause an electric...
  • Page 148 • Maintenance and Service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the device. 5.

  • Page 149: Updating The Device Firmware

    • Maintenance and Service 7.11 Updating the Device Firmware The description in this section refers to the Chromeleon 7 Chromatography Data System. When Updating the device firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version.
  • Page 150 • Maintenance and Service 3. Monitor the Audit Trail of the Instrument Configuration Manager program to see whether the firmware update was successful or failed. 4. Depends on the situation: Situation Action Firmware update Requalification of the device may be required. Refer to successful the release notes.

  • Page 151: Replacing The Doors

    • Maintenance and Service 7.12 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the steps in this section. Parts required Replacement door Follow these steps...
  • Page 152 • Maintenance and Service 3. Slightly tilt the door to the outside, away from the housing, and remove the door. 4. To install the door, align the door with the hinges on the housing. Do not clamp tubing or capillaries between the door and the enclosure. 5.

  • Page 153: Transporting Or Shipping The Device

    • Maintenance and Service 7.13 Transporting or Shipping the Device If you want to transport the device to a new location or if you need to ship the device, first prepare the device for transport, and then move or ship the device as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely.

  • Page 154: Transporting The Device To A New Location

    • Maintenance and Service 4. Remove the flow cell from the device and store it in its packaging. Ensure that you install the flow cell cover to the flow cell opening. Flow cells must be shipped in their original packaging. NOTICE—Sensitive Flow Cells Transporting the detector with a flow cell installed can lead to destruction of the flow cell.

  • Page 155: Shipping The Device

    • Maintenance and Service 4. Set up the device: a) Connect the device and set up flow connections (see Installation (} page 45)). b) Prepare the device for operation (see Preparing the Device for Operation (} page 90)). 5. Before starting an analysis, let the device equilibrate and be sure that it is ready for operation.

  • Page 156: Replacing The Slide-In Module

    • Maintenance and Service 7.14 Replacing the Slide-In Module You can remove the slide-in module from the enclosure of a module for transporting or shipping purposes. The enclosure remains in the system stack. To return a defective module to the factory, install the slide-in module in the enclosure of the replacement module.
  • Page 157 • Maintenance and Service 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to remove the slide-in module properly from the enclosure in the next step.

  • Page 158: Returning The Slide-In Module

    • Maintenance and Service 7.14.2 Returning the Slide-In Module CAUTION—Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel. • Decontaminate all parts of the device that you want to return for repair.
  • Page 159 • Maintenance and Service Tools required Screwdriver, Torx T20 Preparations 1. Unpack the replacement device. Remove the slide-in module from its enclosure as described in Removing the Slide-In Module (} page 156). 2. Verify that the device enclosure into which the slide-in module shall be installed is clean.

  • Page 160: Setting Up The Slide-In Module

    • Maintenance and Service 4. To facilitate tightening the slide-in module in the enclosure, pull out the module a few millimeters (approximately 2 to 5 mm). 5. With the screwdriver, move each captive screw back and forth while pressing inward until the screw slips into the thread. 6.

  • Page 161: Troubleshooting

    • Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device. Diode Array Detectors (VH-D10) Page 161 Operating Manual...

  • Page 162: General Information About Troubleshooting

    • Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the device. TIP For information about operating issues that might occur during the operation of a Vanquish system, refer to the Vanquish System Operating Manual.
  • Page 163 • Troubleshooting Messages in the Instrument Audit Trail are preceded by an icon. The icon identifies the seriousness of the problem. For possible causes and remedial actions, see Messages (} page 164). Firmware Failure If a firmware failure occurred during operation of the module, an exception log has been created about the processes during the firmware failure.

  • Page 164: Messages

    • Troubleshooting Messages The table lists the most frequently observed messages for the device and provides troubleshooting assistance. Each message consists of a code number and a text. The code number is the unique identifier for the problem while the wording may change. Note the following: •...
  • Page 165 • Troubleshooting Message and Code Description and Remedial Action Code 36 Download failed. The firmware download has not been successful. Repeat the download. Code 37 Download firmware The firmware download has not been successful. The firmware is not mismatch. suitable for the detector. Verify that the correct firmware file was selected.
  • Page 166 • Troubleshooting Message and Code Description and Remedial Action Code 3013 Unexpected optics Check whether the flow cell is installed correctly. Flush the flow cell with behavior – flush flow cell with HPLC-grade water. If the error persists, contact Technical Support. water.
  • Page 167 • Troubleshooting Message and Code Description and Remedial Action Code 3133 Holmium validation Repeat the validation. If the message still appears, proceed as follows: failed – specified wavelength • Perform a calibration and repeat the validation again. not found. • Ensure that the flow cell is installed properly. Remove and install the flow cell.
  • Page 168 • Troubleshooting Message and Code Description and Remedial Action Code 3199 Invalid auto-zero The auto-zero data is close to dark current limit (very low intensity). spectral data. Check flow cell. • Make sure that the mobile phase does not show excessive absorption for the selected channel wavelength(s) during autozero.

  • Page 169: Checking The Flow Cell

    • Troubleshooting Checking the Flow Cell When Related messages that require a flow cell check appear in the Audit Trail. Follow these steps 1. Check that the flow cell is installed properly. 2. Remove and re-install the flow cell. 3. Flush the flow cell with pure HPLC-grade water with a flow rate of >1 mL/min for several minutes.

  • Page 170: Resolving Liquid Leaks

    • Troubleshooting Resolving Liquid Leaks When The leak sensor is wet. The leak sensor reports leakage. Parts and additional items required • Replacement part as required • Cloth or tissue Preparations When resolving leakage, observe the safety guidelines and general rules for maintenance and service as presented in Maintenance and Service (} page 111).

  • Page 171: Specifications

    • Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the device. Diode Array Detectors (VH-D10) Page 171 Operating Manual...

  • Page 172: Performance Specifications

    • Specifications Performance Specifications 9.1.1 Detector Specifications The device performance is specified as follows: Type Specification Optical design • Single-beam, reverse-optics design with concave holographic grating • High Numerical Aperture (NA) achromatic optics • 1024-element photodiode array Light source Deuterium lamp for UV and VIS wavelength range (30 W) Wavelength range 190 –...
  • Page 173 • Specifications Type Specification Drift <± 0.5 mAU/h at 230 nm Reference conditions: • Wavelength: as listed; bandwidth 4 nm; reference wavelength not used; slit width: 4 nm • Time constant: 2 sec (response time = 2.2 x time constant as proposed in ASTM). For details, see below this table. •...

  • Page 174: Flow Cell Specifications

    • Specifications Temperature reference conditions for noise, drift and linearity specifications For drift tests, ASTM requires that temperature changes stay below 2 °C/ hour over a period of one hour. The drift specification above is based on these conditions. Larger changes in the ambient temperature will result in a larger drift.

  • Page 175: Physical Specifications

    • Specifications Physical Specifications The physical conditions of the device are specified as follows: Type Specification Range of use Indoor use only Ambient operating 5 °C - 35 °C temperature Ambient storage temperature -20 °C - 45 °C Ambient operating humidity 20% - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing) Operating altitude...
  • Page 176 • Specifications Page 176 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 177: Accessories, Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts.

  • Page 178: General Information

    • Accessories, Consumables and Replacement Parts 10.1 General Information The device must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.

  • Page 179: Ship Kit

    • Accessories, Consumables and Replacement Parts 10.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. Refer to the content list included in the kit for the most recent information about the kit content at the time when the device is shipped.

  • Page 180: Optional Accessories

    • Accessories, Consumables and Replacement Parts 10.3 Optional Accessories Flow cells and Flow cells accessories Item Part No. Back-flush kit for flow cells 6083.4210 Diagnostic cell 6083.0300 LightPipe flow cell, high sensitivity, fused silica, 60 mm 6083.0200B LightPipe flow cell, standard, fused silica, 10 mm 6083.0100B Flushing and injection kit for flow cells, including syringe 6083.4200...

  • Page 181: Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10.4 Consumables and Replacement Parts Lamp Description Part No. UV lamp (deuterium lamp) 6083.1110 Capillaries and tubing for flow cells Description Part No. Viper capillary, I.D. x length 0.10 mm x 300 mm, MP35N, 6083.2405 insulated, connection column compartment - detector Waste line, universal usage 6036.2425 For system capillaries, refer to the Vanquish System Operating Manual.
  • Page 182 • Accessories, Consumables and Replacement Parts Description Part No. Power cord, UK 6000.1020 Power cord, USA 6000.1001 Power cord, Switzerland 6000.1030 Page 182 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 183: Appendix

    • Appendix 11 Appendix This chapter provides additional information about compliance and UV cutoff wavelengths. Diode Array Detectors (VH-D10) Page 183 Operating Manual...

  • Page 184: Compliance Information

    • Appendix 11.1 Compliance Information 11.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. EAC Declaration of Conformity The device has satisfied the requirements for the EAC mark and is compliant with the applicable requirements.

  • Page 185: Weee Compliance

    • Appendix UL/CSA 61010-1 Compliance The label of the NRTL Lab on the device (for example, cTUVus or CSA mark) indicates that the device has satisfied the requirements of the applicable standards. 11.1.2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical &...

  • Page 186: Manual Release History

    • Appendix 11.1.5 Manual Release History Revision Covering VH-D10 2.0a VH-D10 VH-D10 VH-D10 The instructions were prepared in English (original instructions). Other language versions are translations based on the English original instructions. Page 186 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 187: Uv Cutoff Wavelengths Of Solvents

    • Appendix 11.2 UV Cutoff Wavelengths of Solvents The UV cutoff wavelength is the minimum effective wavelength for the measurement. The mobile phase composition affects its UV cutoff wavelength. In general, mobile phases are solvents, such as, water, acetonitrile, methanol, or other substances. They may also contain salts, such as sodium hydroxide (NaOH).
  • Page 188 • Appendix Page 188 Diode Array Detectors (VH-D10) Operating Manual...

  • Page 189: Index

    Index Index cleaning ............ 117 Numerical condensation .......... 50, 54 3D field ............. 98 connection power cord .......... 54 connectors ............ 53 consumables..........  177, 181 accessories ............. 177 cTUVus mark.......... 185 optional ............. 180 ship kit............ 179 acquisition off........... 96 acquisition on ........... 96 data collection rate........ 97, 101 additives ............ 27 guidelines ..........
  • Page 190 Index connect outlet .......... 69 exchange ........ 126, 129, 130 keypad .............  86 flow connections ......... 66 UV ...............  87 flushing............ 126 guidelines ............ 93 identification chip ........ 36 initial installation......... 55 lamp............ 38, 96 install............ 130 turn on............ 92 remove ............ 129 lamp replacement safety........ 46, 85, 93, 115 deuterium lamp (UV)........
  • Page 191 Index requirements power cord .......... 49 NIST compliance.......... 185 response time ........ 97, 101, 102 null balancing ........... 99 optimization .......... 102 restart device after long-term shutdown .. 109 return slide-in module .........  158 operating principle ........... 33 RoHS mark .............  184 operation............ 40, 83 control elements ......... 86 flow cells ............. 93 important parameters......... 96...
  • Page 192 Index site requirements .......... 49 UV button ............ 87 condensation......... 50, 54 UV cutoff wavelengths ........ 187 power considerations...... 49, 50 UV lamp ............ 96 slide-in module.......... 156 turn on............ 92 install............ 159 UV_VIS .............  96 remove ............ 156 return ............ 158 slit width...........  97, 101, 103 Viper fitting system .........
  • Page 193 Index Diode Array Detectors (VH-D10) Page 193 Operating Manual...
  • Page 194 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

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