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Time To Maximum Rate; Figure 10-13 Determining The Time To Maximum Rate - Thermo Scientific Varioskan User Manual

Multimode microplate reader software

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Time to maximum rate

Thermo Fisher Scientific
Window (2...100) - Select the number of consecutive readings to use
for evaluation. The highest reaction rate for each well is calculated using
a sliding window. A window defines how many measurement points
are included in the measurement calculations. The size of this window
is given in the Window parameter box. For example, if the number of
measurements is ten and the Window parameter is three, the system
will calculate the first rate using the measurements 1 to 3, the second
rate using the measurements 2 to 4, and so on up to measurements 8
to 10. The maximum rate will be the maximum value among these
calculated rates.
The time to maximum rate is calculated similarly to the maximum rate, but
the result is reported as the time in seconds from the first reading to the
middle point of the sliding window where the maximum rate occurs instead
of the rate itself. See Figure 10-13.
Figure 10-13. Determining the time to maximum rate
You can define the following settings:
Thermo Scientific SkanIt
Data Processing Parameters
®
®
Software for Varioskan
User Manual
Kinetic calculations
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