Thermo Scientific Varioskan User Manual page 13

Thermo Fisher Scientific
Figures
Figure 1-1 Operation principle ......................................................... 18
Figure 1-2 Varioskan spectral scanning multimode reader .................. 18
Figure 2-1 Computer and mains power supply connectors in the Varioskan
instrument ..................................................................... 38
Figure 3-1 Different parts of the main window .................................. 42
Figure 3-2 Main window for protocol editing ..................................... 43
Figure 3-3 Main window for plate layout editing ............................... 44
Figure 3-4 Main window for results viewing - status reports .............. 45
Figure 3-5> Main window for results viewing - measurement results as a
table .............................................................................. 46
Figure 3-6 Main window for results editing - calculation parameters .... 47
Figure 3-7> Main window for results viewing - calculation results as a
list ................................................................................. 47
Figure 3-8 Main window for run log viewing ..................................... 48
Figure 3-9 Arrow showing the sorting order in lists ............................ 49
Figure 4-1 Session structure ............................................................ 53
Figure 4-2 Tree view in SkanIt Software ........................................... 55
Figure 5-1 Absorption spectra of water ............................................. 73
Figure 6-1 Available execution order types ....................................... 83
Figure 6-2 Measurement after dispense and measurement together with
dispense ........................................................................ 90
Figure 6-3 Interval time, total time and readings in a kinetic loop ........ 91
Figure 7-1 TRF principle ................................................................. 106
Figure 7-2 TRF decay definitions .................................................... 112
Figure 7-3 Dynamic range selections in the FI and TRF measurements . 113
Figure 7-4 Multipoint example: measurement points in a well and their
coordinates in the results ............................................... 115
Figure 7-5 Multipoint selection: center point of a well on a 48-well plate
(A) and on a 6-well plate (B) ........................................... 115
Figure 7-6 Shaking sequence: ON and OFF time and total shaking
time ............................................................................. 119
Figure 7-7 Recommended shaking speed and diameter combinations . 120
Figure 8-1 Plate layout editing window ........................................... 126
Figure 10-1 Dividing measurement results into categories using cut-off
limits ........................................................................... 160
Figure 10-2 Effective dose (ED) determination ................................... 164
Figure 10-3 Spectral peak search: threshold and wavelength area ...... 175
Figure 10-4 Spectral peak search with window count 1 ...................... 175
Figure 10-5 Spectral peak search with window count 2 ...................... 176
Figure 10-6 Definition of the HBW value ........................................... 178
Figure 10-7 HBW calculation in a simple case ................................... 179
Figure 10-8 HBW calculation in a spectrum with two peaks ............... 179
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual 11