Reports - Thermo Scientific Varioskan User Manual

Settings
Instrument
236 Thermo Scientific SkanIt
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Click OK.
4. Select the priming volume of the tip from the drop-down menu; default
is 5/5 µl. The first amount is for the 1,000 µl syringe and the second
for the 5,000 µl syringe. The available range is from 1 to 10 µl for the
1,000 µl syringe and 5 or 10 µl for the 5,000 µl syringe. The tip
priming volume is the same for all the dispensers that use the same
syringe size.
The dispenser dispenses the selected amount of reagent into the tip
priming vessel every time the instrument fills the dispenser syringe.
This makes the volume of the first well equal to that of the others, thus
compensating for the so-called drawback phenomenon. It is
recommended to use the tip priming feature to achieve greater accuracy
when the dispensing volumes are small, for example, 1 to 50 µl.
Note Tip priming is a different procedure from manual priming
that must be performed when reagent bottles are installed into the
dispensers and the dispenser tubes are completely empty.
5. Click OK.

Reports

You can create instrument status reports on the Reports tab. For more
information, see Creating instrument reports" on page 219.
® ®
Software for Varioskan User Manual
Syringe size - Select the volume of the dispenser syringe. 1,000
and 5,000 µl are available; 1,000 µl is the default value. The 5,000
µl syringe is available on request.
Tip size - Select the tip size of the dispenser, either 0.25 or 0.40
mm in diameter; default is 0.40 mm.
Pull-back volume [µl] - Select the pull-back volume for the
dispensing tip in µl. The volume range is 1 to 10 µl for the 1,000
µl syringe and 5 or 10 µl for the 5,000 µl syringe. The default value
is 2 µl for the 1,000 µl syringe and 5 µl for the 5,000 µl syringe.
The pull-back feature prevents liquid droplets from forming onto
the dispensing tip between dispensings. Therefore the liquid is
pulled slightly inward. If set, the dispenser automatically pulls back
the selected amount of liquid into the syringe every time the
instrument dispenses. It is always recommended to use the
pull-back feature when using the 5,000 µl syringe.
Thermo Fisher Scientific