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Data Processing Parameters
Quantitative curve fit
By default, the Average value is always shown in the raw data Table view
if a multipoint measurement has been run. The separate measurement points
for each well are shown in the data list. If another multipoint reduction
calculation type is desired, this can be obtained by adding a Multipoint
calculation step.
For a multipoint, kinetic measurement, the curve shown in the Table view
is based on the middle point of each well. The middle point is calculated
as the coordinate (number of points in the x direction/2, number of points
in the y direction/2). The number is always rounded to the nearest integer
(for example, 3.5 ≈ 4), and it is added from the upper-left corner.
See the example below:
(number of points in the x direction/2, number of points in the y
direction/2) = (12/2, 12/2) = (6, 6)
Quantitative curve fit
Quantitative curve fit is used for quantitative analysis. It calculates the
concentration of the samples based on a standard curve made from a series
of calibrators with known concentrations.
Any dilutions defined for unknowns in the layout are taken into account
in the calculations.
Note Curve fit requires that calibrators have been defined in the
plate layout. The number of calibrators required for different fit
types are given in Table 10-1.
Quantitative curve fit
Select Data Processing > Quantitative Curve Fit, click the
button on
definitions
the Results action panel, or right-click the measurement in the Results tree.
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Thermo Fisher Scientific
Thermo Scientific SkanIt
Software for Varioskan
User Manual
153
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