Select Wavelengths; Kinetic Calculations - Thermo Scientific Varioskan User Manual

Select wavelengths

Kinetic calculations

Thermo Fisher Scientific
Note If you want the calculation to be made only with one
wavelength, make sure you enter the same wavelength for both
Wavelength 1 and Wavelength 2.
Note User-selectable wavelength values are not checked against
real measured values. Therefore, you may select wavelength values
that are not even measured.
• Group - Select the source/assay name from the plate layout.
• Sample - Select the sample/replicate to be used as a base of the ratio
calculation. The spectra of all samples are divided with calued from this
sample.
• Wavelength 1 - Select the starting wavelength to be used in the
scanning.
• Wavelength 2 - Select the end wavelength to be used in the scanning.
Tip If you want to select only one wavelength for further
calculations, select that wavelength as both Wavelength 1 and
Wavelength 2.
Note User-selectable wavelength values are not checked against
real measured values. Therefore, you may select wavelength values
that are not even measured.
Kinetic calculations are used to reduce data from kinetic measurements.
Select Data Processing > Kinetic calculations, click the
Results action panel, or right-click the measurement in the Results tree.
Thermo Scientific SkanIt
Data Processing Parameters
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Software for Varioskan User Manual
Kinetic calculations
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