Defining Multiple Fluorometric Measurements; Fluorometric Spectrum Scanning - Thermo Scientific Varioskan User Manual

Defining multiple
fluorometric measurements
Fluorometric spectrum
scanning
Thermo Fisher Scientific
information, see Defining multiple fluorometric measurements" on
page 101.
When you select the Multiple Measurements check box while defining
the fluorometric measurement parameters, the following dialog opens:
• Add - Enter the new excitation and emission wavelength values in the
Ex [nm] and Em [nm] boxes and then click Add. The new value pair is
added to the list on the left.
• Remove - Select the value pair you want to remove from the list on the
left and click Remove.
• Measurement interval [hh:mm:ss] - Define the time between
measurements with different wavelengths.
In the Fluorometric Spectrum Scanning step, either the emission or the
excitation wavelength is fixed and the other wavelength is scanned.
Select Steps > Fluorometric Scanning or click the
panel.
Thermo Scientific SkanIt

Fluorometric spectrum scanning

button on the action
® ®
Software for Varioskan User Manual
Step Parameters
101