Thermo Scientific Varioskan User Manual page 168

Data Processing Parameters
Effective dose
166 Thermo Scientific SkanIt
When Dilution is selected, you can select a Control or an Unknown
sample.
If you select a Control, the same graph will display all the dilution series.
If you select an Unknown, the graph will only display the dilution series
of that particular sample.
When Concentration is selected, you can select a Control or a
Calibrator.
If you select a Control, the same graph will display all the concentration
series. If you select a Calibrator, the graph will only display the
concentration series of the series where that particular calibrator belongs.
• Use maximum value - You can also select a maximum value to be used
as the ED
with the highest measured value from the selected assay to be the ED
or ED
• ED values - Up to three different ED values can be calculated within
one calculation. The default values are ED
can set any three ED values between 0 and 100.
Note All other selections are equal to quantitative curve fit
selections. In contrast to the Quantitative curve fit function, the
ED determination does not allow extrapolation of the data. All
calculated ED values are between the lowest and highest dilution
given in the source data.
• Fit type - ED values are calculated based on the function generated by
the quantitative curve fit. With this function, the concentration/dilution
values corresponding to the required ED values are solved and listed as
results. Select the curve fit type from the list. The default fit type is
Cubic spline. The curve fit types are explained in Quantitative curve
fit" on page 153.
• Transformation - Select the type of data transformation trailing.
Transformation of statistical data means in brief a change of scale used
to improve the validity of statistical analyses. Logarithmic transformation
is employed when data dynamics is considerable. This facilitates
mathematical adaptations, that is, curve fitting.
Note If the concentration range of the calibrators is more than
four decades, logarithmic transformation should be used to obtain
more precise curve fitting.
• Markers - Select whether the means of the measured calibrator replicates
or individual measurements (replicates) are displayed in the curve.
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Software for Varioskan User Manual
value, which means that the software selects the replicate
100
value.
0
, ED and ED , but you
20 50 80
Thermo Fisher Scientific
100