Data Processing Parameters
Spectral analysis
Spectral maximum
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Thermo Scientific SkanIt
Figure 10-5. Spectral peak search with window count 2
•
Wavelength 1 - Select the starting wavelength for the peak search area.
•
Wavelength 2 - Select the end wavelength for the peak search area.
Note User-selectable wavelength values are not checked against
real measured values. Therefore, you may select wavelength values
that are not even measured.
•
Threshold - Enter the intensity threshold that the peak is required to
exceed. The minimum value is 0.000 units and the maximum is
10,000,000.
•
Window (1 3) - See Figure 10-4 and Figure 10-5.
The spectral maximum seeks the maximum measurement value [Abs] in
each well. You can define a wavelength area for which this calculation is
performed. It is also possible to receive the maximum without defining the
wavelength area or the threshold.
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Software for Varioskan
User Manual
Thermo Fisher Scientific