Thermo Scientific Varioskan User Manual page 188

Data Processing Parameters
Kinetic calculations
186 Thermo Scientific SkanIt
• Reaction - Define whether the reaction is supposed to produce
increasing or decreasing signal levels. There are three options for this
setting:
• Undefined (default) - The reaction can be increasing, decreasing
or both (for example, first increase and then decrease, that is,
peaking). The software does not produce any warnings in either
case. It searches for the absolute maximum rate. If the highest
absolute rate value is increasing, it is shown as a positive number.
If the maximum rate is decreasing, it will be presented with the
prefix "-".
• Increasing - The software searches for the maximum increasing
rate. The rate is shown as a positive number. If no increasing rate
is found, the result is NaN" (not a number).
• Decreasing - The software searches for the maximum decreasing
rate. The rate is shown as a positive number. If no decreasing rate
is found, the result is NaN" (not a number).
• Kinetic rate - Select to view the results in either seconds (s) or minutes
(min). The kinetic rate is always calculated per second but if needed,
the time is converted to minutes after the calculations.
• Ignore first (n) readings - Ignores a defined number of points
(readings), counted from the first reading.
• Ignore last (n) readings - Ignores a defined number of points (readings),
counted from the last reading.
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Software for Varioskan User Manual Thermo Fisher Scientific