Thermo Scientific Varioskan User Manual page 132

Plate Layouts
Editing a plate layout
130 Thermo Scientific SkanIt
a blank group with the same number of samples as in the target group
is constructed. Each sample has its own corresponding blank sample
from the blank group. The average of the blank sample replicates is
calculated and then subtracted from the unknown samples on a
sample-by-sample basis when blank subtraction is performed. Not
available for Blanks or Empty wells.
Dilution/Concentration:
• Dilution/Concentration - Enter the reagent volume of the first sample
in the dilution or concentration series. Dilution is available for
Unknowns, and Concentration for Controls and Calibrators.
• Unit - Enter the unit for the dilution or concentration volume.
Tip In some keyboards, you can enter the character "µ" by pressing
AltGr+M.
• Generate series - Tick the check box to generate concentration and
dilution series. The Concentrations or Dilutions dialog opens (see
Adding a concentration or dilution series" on page 131). Not available
for Blanks or Empty wells.
Buttons:
• Clear All - Clicking this button will clear all wells.
• Load IDs - You can load a text file (.txt) containing the IDs of the
unknown samples. The unknowns will be named according to the IDs
in the file. The file must only contain sample names (no spaces allowed)
separated by line breaks (see Example 8-1, Text file for defining names
for unknown samples" below). The Load IDs button is only available
for Unknowns.
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