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Thermo Scientific LTQ Series Hardware Manual

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LTQ Series
Hardware Manual
97055-97072 Revision C
June 2012

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  • Page 1 LTQ Series Hardware Manual 97055-97072 Revision C June 2012...
  • Page 2 © 2012 Thermo Fisher Scientific Inc. All rights reserved. Automatic Gain Control, Foundation, Ion Max, LTQ XL, Velos, Velos Pro, and ZoomScan are trademarks, and Accela, LTQ, LXQ, Thermo Scientific, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States.
  • Page 3 Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards, replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives. Regulatory compliance results for the following Thermo Scientific products: • LXQ Mass Spectrometer (February 2005) •...
  • Page 4 LTQ XL Mass Spectrometer (September 2006) EMC Directive 89/336/EEC EMC compliance has been evaluated by TÜV Rheinland of North America, Inc. EN 55011: 1998, A1: 1999, A2: 2002 EN 61000-4-3: 2002 EN 61000-3-2: 1995, A1: 1998, A2: 1998, A14: 2000 EN 61000-4-4: 1995, A1: 2001, A2: 2001 EN 61000-3-3: 1995, A1:2001 EN 61000-4-5:1995, A1: 2001...
  • Page 5 MALDI LTQ XL System (August 2007) EMC Directive 2004/108/EC EMC compliance has been evaluated by TÜV Rheinland of North America, Inc. EN 55011: 1998, A1: 1999, A2: 2002 EN 61000-4-3: 2002 EN 61000-3-2: 2000 EN 61000-4-4: 1995, A1: 2000, A2: 2001 EN 61000-3-3: 1995, A1: 2001 EN 61000-4-5: 2001 EN 61326-1: 1998, A2: 2001, A3: 2003...
  • Page 6 LTQ Velos/ETD System (November 2008) EMC Directive 2004/108/EEC EMC compliance has been evaluated by TÜV Rheinland of North America, Inc. EN 61326-1: 2006 EN 61000-4-4: 2004 EN 55011: 2007 EN 61000-4-5: 2005 EN 61000-3-2: 2006 EN 61000-4-6: 2007 EN 61000-3-3: 2005 EN 61000-4-11: 2004 EN 61000-4-2: 2001 FCC Part 15: 2007...
  • Page 7 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument.
  • Page 8 Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument. Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment.
  • Page 9 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 10 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
  • Page 11 CAUTION Symbol CAUTION VORSICHT ATTENTION PRECAUCION AVVERTENZA Electric Shock: This instrument uses Elektroschock: In diesem Gerät werden Choc électrique: L’instrument utilise des Descarga eléctrica: Este instrumento Shock da folgorazione. L’apparecchio è high voltages that can cause personal Hochspannungen verwendet, die tensions capables d’infliger des blessures utiliza altas tensiones, capaces de alimentato da corrente ad alta tensione...
  • Page 12 CAUTION Symbol CAUTION Electric Shock: This instrument uses high voltages that can cause personal injury. Before servicing, shut down the instrument and disconnect the instrument from line power. Keep the top cover on while operating the instrument. Do not remove protective covers from PCBs. Chemical: This instrument might contain hazardous chemicals.

  • Page 13: Table Of Contents

    LTQ Series Mass Spectrometers ........
  • Page 14 Checking the ESI Fused-Silica Sample Tube for Elongation ... . . 48 After Operating the LTQ Series System ......48 Flushing the Sample Transfer Line, Sample Tube, and API Probe.
  • Page 15 Index ............. . .141 Thermo Scientific LTQ Series Hardware Manual...
  • Page 17 LTQ Series front panel LEDs ........
  • Page 18 ..........118 Figure 82. Diagnostics dialog box showing the Set Device list ..... 119 xviii LTQ Series Hardware Manual Thermo Scientific...
  • Page 19 Diagnostics dialog box showing the general test page ....126 Figure 89. Diagnostics dialog box showing the system evaluation tools page ..127 Thermo Scientific LTQ Series Hardware Manual...
  • Page 21 Preface The LTQ Series Hardware Manual describes the modes of operation and hardware components. It also provides the instrument’s cleaning and maintenance procedures for the following LTQ™ Series mass spectrometers (MSs): • LXQ™, a single-segment 2D linear ion trap mass spectrometer •...

  • Page 22: Preface

    Preface Related Documentation In addition to this guide, Thermo Fisher Scientific provides the following documents accessible from the data system computer for the LTQ Series mass spectrometers: • LTQ Series Preinstallation Requirements Guide • LTQ Series Getting Connected Guide • LTQ Series Getting Started Guide •...

  • Page 23: Getting A New License Code

    7. Click Close. Note Do not click Reset in the LTQ License dialog box. If you inadvertently click Reset, you must reopen the LTQ License dialog box to copy a new license key for step Thermo Scientific LTQ Series Hardware Manual xxiii...

  • Page 24: Installing A New License Code

    2. In the License box, paste a copy of the new license number from the e-mail message and click Set. Example license number 3. Click OK when the following message appears: The new license number has been set. xxiv LTQ Series Hardware Manual Thermo Scientific...

  • Page 25: Safety And Special Notices

    Note Highlights information of general interest. Tip Highlights helpful information that can make a task easier. Thermo Scientific LTQ Series Hardware Manual...

  • Page 26: Contacting Us

    Preface Table 1 lists additional caution-specific symbols that appear in the LTQ Series Hardware Manual. Table 1. Caution-specific symbols and their meanings Symbol Meaning Chemical: Hazardous chemicals might be present in the instrument. Wear gloves when handling carcinogenic, corrosive, irritant, mutagenic, or toxic chemicals.
  • Page 27 Customer Manuals in the left margin of the window.  To suggest changes to documentation or to Help • Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62. • Send an e-mail message to the Technical Publications Editor at techpubs-lcms@thermofisher.com. Thermo Scientific LTQ Series Hardware Manual xxvii...

  • Page 29: Introduction

    Introduction The LTQ Series linear ion trap mass spectrometers are part of the Thermo Scientific family of mass spectrometers. Note The “Glossary” page 137 defines some of the terms used in this manual. Contents • LTQ Series Mass Spectrometers •...
  • Page 30 Introduction LTQ Series Mass Spectrometers Velos Pro Model Velos Pro is a dual-cell 2D linear ion trap mass spectrometer that includes a syringe pump, a divert/inject valve, and an Ion Max™ ion source. This instrument has an improved ion detection system and other added features.
  • Page 31 Introduction LTQ Series Mass Spectrometers LTQ XL Model LTQ XL is a three-segment 2D linear ion trap mass spectrometer that includes a syringe pump, a divert/inject valve, and an Ion Max ion source. Model LXQ is a single-segment 2D linear ion trap mass spectrometer that includes a syringe pump, a divert/inject valve, and an Ion Max-S ion source.

  • Page 32: Overview Of An Lc/Ms Analysis

    As the separated chemical components exit the LC column, they pass through a transfer line and enter the LTQ Series mass spectrometer where they are ionized and analyzed. As the mass spectrometer analyzes the ionized components and determines their mass-to-charge (m/z) ratios, it sends a data stream to the data system computer.
  • Page 33 The data system serves as the user interface to the mass spectrometer, autosampler, LC, and syringe pump. Refer to the data system Help for more information about the LTQ Series data processing and instrument control software.

  • Page 35: Chapter 2 Functional Description

    Overview Figure 1 shows a functional block diagram of an LTQ Series system that uses either a liquid chromatograph with an autosampler or the syringe pump as the inlet. A sample transfer line connects the LC to the mass spectrometer. The autosampler and LC are usually installed to the left of the mass spectrometer.

  • Page 36: Figure 1. Functional Block Diagram Of An Ltq Series System

    The signal then passes on to the data system for further processing, storage, and display. The data system provides the primary mass spectrometer user interface. Figure 1. Functional block diagram of an LTQ Series system Inlet Mass spectrometer...

  • Page 37: Syringe Pump

    Liquid flows out of the syringe needle and into the sample transfer line as the plunger is depressed. A syringe holder holds the syringe in place. For instructions about setting up the syringe pump, refer to the LTQ Series Getting Started Guide. Syringe Pump On/Off Button...

  • Page 38: Divert/Inject Valve

    Divert/Inject Valve The divert/inject valve is located on the front of the LTQ Series mass spectrometer to the left of the API source. You can configure (plumb) the divert/inject valve in one of two ways: as a divert valve for direct infusion, high-flow infusion, or LC/MS experiments;...

  • Page 39: Divert Valve Positions

    Tune Plus. Solvent flow from the LC pump backflushes sample out of the sample loop and then out of the valve through port 3 toward the detector. Thermo Scientific LTQ Series Hardware Manual...

  • Page 40: Mass Spectrometer

    Inject position Mass Spectrometer The LTQ Series mass spectrometer provides sample ionization and mass analysis of injected samples or samples eluted from a liquid chromatograph. The mass spectrometer uses a linear ion trap mass analyzer with an ion source external to the mass analyzer.

  • Page 41: Leds

    Functional Description Mass Spectrometer LEDs Figure 7 shows the LEDs on the front panel of the LTQ Series mass spectrometer with their descriptions listed in Table Figure 7. LTQ Series front panel LEDs Power Vacuum Communication System Scanning Table 4. LTQ Series status LED descriptions...

  • Page 42: Power Panel

    (Peripheral Control), an Ethernet 100Base-T connection from the mass spectrometer to the data system computer, and a system Reset button. The power panel accepts line power, filters it, and provides it to various components of the mass spectrometer. LTQ Series Hardware Manual Thermo Scientific...

  • Page 43: Api Source

    LTQ Series Getting Started Guide. Ion Source Interface The ion source interface for the LTQ Series mass spectrometer consists of the components of the API source that are held under vacuum (except for the atmospheric pressure side of the sweep cone) in a vacuum chamber that the one or two forepumps evacuate.

  • Page 44: Figure 10. Lxq Ion Source Interface (Cross Section)

    11). The tube lens and skimmer mount to the ion source interface cage. Figure 10. LXQ ion source interface (cross section) Vent prevent ball Tube lens Ion sweep cone Skimmer Spray cone Ion transfer tube (immediately behind the sweep cone) Heater block LTQ Series Hardware Manual Thermo Scientific...

  • Page 45: Figure 11. Ltq Xl Ion Source Interface (Cross Section)

    (CID), in the ion transfer tube–skimmer region decreases sensitivity. Therefore, when you tune the LXQ or LTQ XL mass spectrometer, adjust the tube lens offset voltage to maximize sensitivity by balancing desolvation with fragmentation. Thermo Scientific LTQ Series Hardware Manual...

  • Page 46: Figure 12. Skimmer And Tube Lens (Back View)

    The S-lens and exit lens mount to the ion source interface cage. Figure 13. Velos Pro ion source interface (cross section) Vent prevent ball Ion sweep cone S-lens Exit lens Spray cone (directly behind the sweep cone) Ion transfer tube Heater block LTQ Series Hardware Manual Thermo Scientific...

  • Page 47: Figure 14. Velos Pro Exit Lens And S-Lens (Back View)

    MP00 rf lens. During the tune procedure, the Velos Pro determines the mass-dependent rf amplitudes for optimum transmission of ions through the lens. Figure 14. Velos Pro exit lens and S-lens (back view) Exit lens S-lens Thermo Scientific LTQ Series Hardware Manual...

  • Page 48: Ion Optics

    17). An rf voltage applied to the rods creates an electric field that guides the ions along the axis of the multipole. The MP0 offset voltage increases the translational kinetic energy of ions that emerge from MP00. LTQ Series Hardware Manual Thermo Scientific...

  • Page 49: Figure 16. Mp0 For The Lxq And Ltq Xl

    18). This feature helps prevent the neutral molecules from entering the mass analyzer chamber. Figure 16. MP0 for the LXQ and LTQ XL Figure 17. MP0 for the LTQ Velos Figure 18. MP0 with a beam stop post for the Velos Pro Thermo Scientific LTQ Series Hardware Manual...

  • Page 50: Figure 19. Mp1 Ion Optics

    For positive beams, the split gate lens opens at –90 V and closes at 50 V. Figure 19. MP1 ion optics Split gate lens LXQ and LTQ XL Velos Pro LTQ Series Hardware Manual Thermo Scientific...

  • Page 51: Mass Analyzer

    The LXQ rods have one section and the LTQ XL rods have three sections. Figure 20 shows the basic design of the linear ion trap. Figure 20. LTQ XL linear ion trap rod assembly Back section Center section Front section Thermo Scientific LTQ Series Hardware Manual...
  • Page 52 Ions consecutively move into resonance with the resonance ejection ac voltage. When an ion approaches resonance, it moves farther away from the center of the mass analyzer and is subsequently ejected. LTQ Series Hardware Manual Thermo Scientific...
  • Page 53 As the main rf voltage increases, ions of greater and greater mass-to-charge ratios become unstable and eject through the slots in the exit rods. Most of these ions are focused toward the ion detection system where they are detected. Figure 21 illustrates this process. Thermo Scientific LTQ Series Hardware Manual...

  • Page 54: Ion Detection Systems

    Ion Detection Systems The LTQ Series mass spectrometer has a high-sensitivity, off-axis ion detection system. The LXQ has one electron multiplier, and the LTQ XL and Velos Pro have two detectors.

  • Page 55: Vacuum System

    The vacuum system evacuates the region around the stack, ion optics, mass analyzer, and ion detection system. The vacuum system includes the following components (Figure 22): • Vacuum Manifold • Turbomolecular Pump • Forepumps • Convectron Gauge • Ion Gauge Thermo Scientific LTQ Series Hardware Manual...

  • Page 56: Figure 22. Functional Block Diagram Of The Vacuum System

    Atmospheric Source MP00 Analyzer pressure region rf lens ion guide region Sample region region region Sample tube inlet device gauge Triple-inlet Convectron turbomolecular pump gauge Exhaust Vent Air filter valve Forepump Foreline LTQ Series Hardware Manual Thermo Scientific...

  • Page 57: Figure 23. Lxq Vacuum Manifold With Top Cover Plate And Turbomolecular Pump

    The vacuum manifold is divided into four chambers by three baffles. Figure 23. LXQ vacuum manifold with top cover plate and turbomolecular pump Top cover plate Vacuum manifold Turbomolecular pump Thermo Scientific LTQ Series Hardware Manual...

  • Page 58: Figure 24. Turbomolecular Pump

    Power to the turbomolecular pump automatically shuts off if the turbomolecular pump’s temperature becomes too high. The turbomolecular pump sends status information, such as its temperature or rotational speed, to the data system computer. LTQ Series Hardware Manual Thermo Scientific...

  • Page 59: Inlet Gases Hardware

    When power to the vent valve solenoid shuts off for more than a very brief period of time, the vent valve opens and the manifold vents to filtered air. Thermo Scientific LTQ Series Hardware Manual...

  • Page 60: Cooling Fans

    The exhaust air exits from the vent slots on the sides of the mass spectrometer. CAUTION To ensure safety and proper cooling, always operate the mass spectrometer with its covers in place. This is also necessary to comply with product safety and electromagnetic interference regulations. LTQ Series Hardware Manual Thermo Scientific...

  • Page 61: System Shutdown, Startup, And Reset

    System Shutdown, Startup, and Reset Many maintenance procedures for the LTQ Series mass spectrometer require that the system be shut down completely. If the system is not used for 12 hours or more, you can place the mass spectrometer in standby mode. If the system is not used for 2 months or more, you can shut down the mass spectrometer completely.

  • Page 62: Opening The Tune Plus Window

    • On the Windows taskbar, choose Start > All Programs > Thermo Instruments > LTQ > Tune, where model is your specific LTQ Series model. Note For LTQ Series version 2.5.0 or earlier, choose Start > All Programs > Xcalibur > Tune.

  • Page 63: Placing The Mass Spectrometer In Standby Mode

    Placing the Mass Spectrometer in Standby Mode You do not need to shut down the LTQ Series mass spectrometer completely if you are not going to use it for 12 hours or more. Instead, place the mass spectrometer in standby mode.

  • Page 64: Turning On The Mass Spectrometer

    Shutting Down the Mass Spectrometer Completely Shut down the LTQ Series mass spectrometer completely only if it is not in use for an extended period of time or if it must be shut down for maintenance or service. You do not need to shut down the system completely if you are not going to use it for a short period of time, such as overnight or through the weekend.

  • Page 65: Starting The System After A Complete Shutdown

    3. Shut down the data system computer, and turn off the monitor and printer, if provided. 4. If provided, turn off the autosampler by using its On/Off switch. Starting the System after a Complete Shutdown To power up the LTQ Series mass spectrometer after it has been shut down completely, follow these procedures: •...

  • Page 66: Starting The Data System

    • After several more seconds, the Communication LED turns green to indicate that the data system and the mass spectrometer have established a communication link. Make sure that the instrument console window is active. The data system transfers operational software to the mass spectrometer. LTQ Series Hardware Manual Thermo Scientific...

  • Page 67: Starting The Autosampler

    Turn on the autosampler by using its on/off power switch. If necessary, configure the autosampler. For procedures for placing sample vials, preparing solvent and waste containers, installing syringes, and so on, refer to the autosampler manual. The LTQ Series Getting Connected Guide provides autosampler connection procedures.

  • Page 68: Resetting Tune And Calibration Parameters

    Resetting Tune and Calibration Parameters You can reset the LTQ Series mass spectrometer tune and calibration parameters to their default values at any time. This feature is useful if you have manually set some parameters that resulted in less than optimum performance.

  • Page 69: Restarting The Data System

    • Turn off the Main Power switch, which removes all power to the mass spectrometer, including the vacuum system. Table 5 summarizes the On/Off status of mass spectrometer components, voltages, and gas flows. Thermo Scientific LTQ Series Hardware Manual...
  • Page 70 API tube lens Ion optics lens Ion optics multipoles dc offset voltages Mass analyzer, dc offset voltage Power supply, 300 Vdc Gas, sweep (LTQ Series 2.7 SP1 or later) API ion transfer tube heater Fan, rf coil Gauge, Convectron Gauge, ion...
  • Page 71 Electronics Main Power switch, mode mode Mass spectrometer component service switch, Off (O) position Service Mode position Fan, turbomolecular pump Fans, electronics tower Forepump Power supply, PS2 Turbomolecular pump Turbomolecular pump controller Gas, helium Thermo Scientific LTQ Series Hardware Manual...

  • Page 73: Chapter 4 Daily Operation

    Tune parameters are instrument parameters that affect the intensity of the ion signal. You calibrate the LTQ Series system by using the ESI calibration solution about once every month. Do some calibrations, such as for the electron multiplier gain, more often.

  • Page 74: Before Operating The Ltq Series System

    If there is a major air leak, the system does not pump down to sufficient levels to turn on the system. CAUTION For proper performance, operate the LTQ Series system at the proper vacuum levels. Operating the system with poor vacuum levels can cause reduced sensitivity, tuning problems, and reduced electron multiplier life.
  • Page 75 Daily Operation Before Operating the LTQ Series System 3. Check the Convectron Gauge Pressure readback. This readback displays the current pressure in the ion transfer tube–skimmer and foreline region. 4. Compare the current values of the pressures in the vacuum manifold with the following values.

  • Page 76: Checking The Gas Supplies

    5560–26700 L (196–943 ft For more information about gas requirements, including those for a MALDI LTQ XL system and the ETD reagent carrier gas for the LTQ XL/ETD system, refer to the LTQ Series Preinstallation Requirements Guide.

  • Page 77: Flushing The Sample Transfer Line, Sample Tube, And Api Probe

    Daily Operation After Operating the LTQ Series System Flushing the Sample Transfer Line, Sample Tube, and API Probe Flush the sample transfer line, sample tube, and API probe at the end of each work day (or more often if you suspect they are contaminated) with a 50:50 methanol/water solution from the LC system through the API source at a flow rate of 200–400 μL/min for approximately...

  • Page 78: Figure 28. Esi Source Dialog Box

    Daily Operation After Operating the LTQ Series System g. Click OK. h. Go to step 5. To flush the ESI source: a. Choose Setup > ESI Source to open the ESI Source dialog box (Figure 28). Figure 28. ESI Source dialog box b.

  • Page 79: Cleaning The Ion Sweep Cone, Spray Cone, And Ion Transfer Tube

    CAUTION When the ion transfer tube is installed, do not flush it with cleaning solution, which flushes the residue into the mass spectrometer. For detailed instructions, refer to Chapter 8 in the LTQ Series Getting Started Guide. Purging the Oil in the Forepump Purge (decontaminate) the oil in the forepump daily to remove water and other dissolved chemicals, which can cause corrosion and decrease the lifetime of the forepump.

  • Page 80: Emptying The Solvent Waste Container

    Daily Operation After Operating the LTQ Series System Emptying the Solvent Waste Container Check the solvent level in the solvent waste container daily. If necessary, empty the container and dispose of the solvent waste in accordance with local and national regulations.

  • Page 81: Maintenance

    Maintenance The performance of the LTQ Series mass spectrometer depends on the maintenance of all parts of the instrument. You are responsible for maintaining the system properly by performing the system maintenance procedures on a regular basis. Note Unless otherwise noted: •...
  • Page 82 Maintenance Table 6 lists the maintenance procedures and their recommended frequency. Table 6. LTQ Series mass spectrometer maintenance procedures and frequency Component Procedure Frequency Location API source Flush (clean) the sample transfer Daily page 49 line, sample tube, and API probe.

  • Page 83: Tools And Supplies

    Maintenance Tools and Supplies Tools and Supplies The LTQ Series mass spectrometer requires very few tools to perform routine maintenance procedures. You can remove and disassemble many of the components by hand. Table 7 lists the necessary tools, equipment, and chemicals for maintaining the API source, ion guides, mass analyzer, and ion detection system.

  • Page 84: Ion Source Probe Maintenance

    Chamois-tipped swabs Magnification device Gloves, lint-free and powder-free 7/64 in. hex driver Kimwipes or lint-free industrial tissues 5/16 in. open-end wrench Methanol, LC/MS-grade 3/8 in. open-end wrench Nitrogen gas 1/2 in. open-end wrench Water, LC/MS-grade LTQ Series Hardware Manual Thermo Scientific...

  • Page 85: Esi Probe Maintenance

    To remove contamination, use a 50:50 solution of LC/MS-grade methanol and LC/MS-grade water, and flush the solution from the LC system through the API source for approximately 15 minutes. For instructions, see “Flushing the Sample Transfer Line, Sample Tube, and API Probe” page Thermo Scientific LTQ Series Hardware Manual...

  • Page 86: Figure 29. Two-Piece Fingertight Fitting And Loosened Sample Inlet Adapter Fitting

    2. Remove the ESI probe from the API source housing. For instructions, refer to Chapter 2, “Setting Up the API Source,” in the LTQ Series Getting Started Guide. 3. Loosen the red fitting of the two-piece fingertight fitting that secures the position of the...
  • Page 87 2. Remove the ESI probe from the API source housing. For instructions, refer to Chapter 2, “Setting Up the API Source,” in the LTQ Series Getting Started Guide. 3. Unscrew the red fitting of the two-piece fingertight fitting from the sample inlet adapter fitting and remove the sample tube from the ESI probe.

  • Page 88: Figure 30. Esi Probe With The Nozzle Removed

    ESI nozzle, and the HV socket O-rings. In addition, the ESI nozzle and manifold occasionally require cleaning. For Field Service use only Part number ESI 26-gauge spray needle 00950-00990 ESI needle seal 00950-00952 ESI nozzle O-ring, Viton™, 0.676 in. ID 00107-05710 ESI high-voltage socket O-ring 00107-02550 LTQ Series Hardware Manual Thermo Scientific...

  • Page 89: Figure 31. Esi Nozzle And O-Ring

    ESI manifold. Tapping the manifold against a hard surface can also help to dislodge the resistor and battery contact. 4. If necessary, replace the needle seal, the 26-gauge spray needle, or both. 5. Reassemble the ESI probe (see steps 1–6 “To assemble the ESI probe” page 62). Thermo Scientific LTQ Series Hardware Manual...

  • Page 90: Figure 32. Front End Of The Esi Probe (Exploded View)

    4. Make sure that the 0.676 in. ID O-ring is in the precut groove on the ESI nozzle (Figure 31 page 61). 5. Thread the ESI nozzle over the needle and into the ESI manifold. Slightly wet the nozzle threads with LC/MS-grade methanol for lubrication. LTQ Series Hardware Manual Thermo Scientific...
  • Page 91 (for the 1/16 in. OD high-pressure tubing) (Upchurch Scientific F-300) Fitting, fingertight, red, one wing 00101-18195 (for the 1/16 in. OD high-pressure tubing) (Upchurch Scientific F-200) Fitting, grounding union, stainless steel, 00101-18182 0.010 in. thru-hole (Upchurch Scientific U-435) Thermo Scientific LTQ Series Hardware Manual...

  • Page 92: Figure 33. Fused-Silica Sample Tube Inserted Through The Front Of The Esi Probe

    The remaining length of the sample tube protrudes from the ESI probe sample inlet at the back of the probe (Figure 33). Figure 33. Fused-silica sample tube inserted through the front of the ESI probe ESI needle Fused-silica sample tube LTQ Series Hardware Manual Thermo Scientific...

  • Page 93: Figure 34. Natural Peek Safety Sleeve Installed In The Sample Inlet (Esi Probe)

    Slide the natural PEEK ferrule with the tapered end facing the sample inlet onto the safety sleeve. b. Slide the red, one-wing fingertight fitting with the threaded end facing the sample inlet onto the safety sleeve. Thermo Scientific LTQ Series Hardware Manual...

  • Page 94: Figure 36. Fingertight Fittings Installed Over The Safety Sleeve (Esi Probe)

    To prevent the sample tube from slipping through the grounding union thru-hole and out the other end, connect a fingertight fitting and red PEEK tubing to the other end of the grounding union. LTQ Series Hardware Manual Thermo Scientific...

  • Page 95: Figure 38. Safety Sleeve Connection To The Grounding Union (Esi Probe)

    • For flow rates greater than 100 μL/min, make sure that the sample tube is flush with the ESI needle tip or recessed inside the ESI needle by less than 1 mm (Figure 41). Thermo Scientific LTQ Series Hardware Manual...

  • Page 96: Figure 40. Adjusting The Sample Tube Position (Esi Probe)

    PEEK fitting and reposition the sample tube. Figure 41. Sample tube position for flow rates greater than 100 μL/min (ESI probe) 26-gauge electrospray needle Nozzle Sample tube Sample inlet end of ESI probe < 1 mm LTQ Series Hardware Manual Thermo Scientific...

  • Page 97: Hesi-Ii Probe Maintenance

    To support flow rates of 5–2000 μL/min, Thermo Fisher Scientific provides two metal needle inserts for the HESI-II probe. The difference between the two inserts is the size of the metal needle and supporting ferrule. For part numbers, see “Ion Source Probes and Parts” page 132. Thermo Scientific LTQ Series Hardware Manual...

  • Page 98: Figure 42. Components Of The Metal Esi Needle Insert

    35) and let it cool to room temperature. 2. Remove the HESI-II probe from the API source housing. For instructions, refer to Chapter 2, “Setting Up the API Source,” in the LTQ Series Getting Started Guide. 3. Unscrew the fingertight fitting from the sample inlet port.

  • Page 99: Figure 44. Hesi-Ii Probe Disassembled (Exploded View)

    Metal needle 1.5 mm 7. Place the end cover on the probe body, insert the two hex socket-head screws into the end cover, and then tighten them by using the 3 mm hex driver. Thermo Scientific LTQ Series Hardware Manual...

  • Page 100: Apci Probe Maintenance

    LC pump on while the probe cools to room temperature. Figure 46 Figure 47 show the major components of the APCI probe. Figure 46. APCI probe Guide pin Vaporizer heater cable socket Flat side on APCI nozzle head Retention APCI nozzle flanges assembly LTQ Series Hardware Manual Thermo Scientific...

  • Page 101: Figure 47. Apci Probe (Cross Section)

    To remove contamination, use a 50:50 solution of LC/MS-grade methanol and LC/MS-grade water, and flush the solution from the LC system through the API source for approximately 15 minutes. For instructions, see “Flushing the Sample Transfer Line, Sample Tube, and API Probe” page Thermo Scientific LTQ Series Hardware Manual...

  • Page 102: Figure 48. Sample Inlet End Of The Apci Probe Showing Locked And Unlocked

    4. Place the nozzle assembly on the clean, lint-free work surface. Cleaning the APCI Nozzle For Field Service use only Part number O-ring, silicone, 0.312 in. ID, 1/16 in. thick 00107-04500 O-ring, Viton, 0.500 in. ID, 1/16 in. thick 00107-05600 LTQ Series Hardware Manual Thermo Scientific...

  • Page 103: Figure 49. Apci Probe Nozzle Assembly

    • If you do not want to replace the APCI sample tube, follow the procedure “To reassemble the APCI probe” page • If you want to replace the sample tube, follow the procedure “To remove the APCI sample tube” page Thermo Scientific LTQ Series Hardware Manual...

  • Page 104: Figure 50. 3/8 In. Open-End Wrench On The Apci Flange Fitting

    Figure 51. APCI sample tube connection Sample tube 10-32 male nut, natural PEEK APCI nozzle 0.239 in. ID Viton O-ring 0.016 in. ID ferrule, natural PEEK APCI flange fitting 4. Discard the used sample tube. LTQ Series Hardware Manual Thermo Scientific...

  • Page 105: Figure 52. Sample Tubing Connection To The Apci Flange Fitting

    7. Using the fused-silica cutting tool, cut the sample tube approximately 1 mm past the tip of the APCI nozzle (Figure 53). Figure 53. Sample tube extending past the APCI nozzle tip APCI flange fitting 1 mm APCI nozzle tip Thermo Scientific LTQ Series Hardware Manual...

  • Page 106: Ion Source Interface Maintenance

    • Prepare a clean work surface by covering the area with lint-free paper. • Put on a new pair of lint- and powder-free gloves before starting each of these removal, cleaning, and reinstallation procedures. LTQ Series Hardware Manual Thermo Scientific...

  • Page 107: Removing The Ion Source Interface

    Venting the mass spectrometer can take several minutes. 2. Unplug the mass spectrometer’s power supply cord from the electrical outlet. 3. Remove the API source housing. For instructions, refer to Chapter 2, “Setting Up the API Source,” in the LTQ Series Getting Started Guide. Thermo Scientific...

  • Page 108: Cleaning The Skimmer And Tube Lens On The Lxq And Ltq Xl Mass Spectrometers

    CAUTION Do not scratch the outside of the skimmer cone. To clean the skimmer and tube lens, follow these procedures: To remove and clean the tube lens and skimmer To reinstall the tube lens and skimmer, on page 83 LTQ Series Hardware Manual Thermo Scientific...

  • Page 109: Figure 55. Skimmer, Tube Lens, And Ion Source Interface Cage For The Lxq And

    Ball plunger holes (for one side) Tube lens Contact ring Ion source interface cage support Skimmer Ball plunger holes (for one side) LTQ XL Tube lens Contact ring Ion source interface support outer cage Skimmer Thermo Scientific LTQ Series Hardware Manual...
  • Page 110 5. Using a magnification device, inspect the components for any residual lint or particulates. Note Inspect the inside surfaces and edges to confirm that no lint or particulates are present. Use tweezers or a similar tool to remove the lint or particulate. LTQ Series Hardware Manual Thermo Scientific...

  • Page 111: Cleaning The Exit Lens And S-Lens On The Velos Pro

    No tools are needed to remove or install these components. To clean the exit lens and S-lens, follow these procedures: To remove the exit lens and S-lens To clean the exit lens and S-lens To reinstall the S-lens and exit lens Thermo Scientific LTQ Series Hardware Manual...

  • Page 112: Figure 57. Exit Lens, S-Lens, And Ion Source Interface Cage For The Velos Pro

    To clean the areas that you cannot reach with the chamois-tip swab, use the 6000 grit MICRO-MESH polishing swabs. 3. For the exit lens, clean the bore by using the 6000 grit MICRO-MESH polishing swabs. LTQ Series Hardware Manual Thermo Scientific...

  • Page 113: Cone

    To clean the spray cone and O-ring To clean the ion sweep cone To reinstall the ion source interface, on page 91 For Field Service use only Part number O-ring, Vespel™, 0.325 in. ID 97055-20442 Ion transfer tube page 133. Thermo Scientific LTQ Series Hardware Manual...

  • Page 114: Figure 58. Vacuum Dialog Box

    Choose Setup > Inlet Direct Control to open the Inlet Direct Control dialog box (Figure 59). Figure 59. Inlet Direct Control dialog box for an Accela 1250 Pump Click this button to turn off the pump. c. Click the Pump Off or Stop Pump button. LTQ Series Hardware Manual Thermo Scientific...

  • Page 115: Figure 60. Ion Sweep Cones

    3. After cooling to room temperature, remove the API source housing from the front of the mass spectrometer. For instructions, refer to Chapter 2, “Setting Up the API Source,” in the LTQ Series Getting Started Guide. 4. Grasp the outer ridges of the ion sweep cone and pull it off of the API cone seal. If necessary, first loosen the ball plungers by using a small slotted screwdriver.

  • Page 116: Figure 61. Ion Transfer Tube Removal Tool

    4. Reinstall the ion transfer tube into the heater block, as follows: a. Using the custom removal tool, rotate the ion transfer tube clockwise as you insert it. b. Continue turning the tube until it is fingertight. LTQ Series Hardware Manual Thermo Scientific...

  • Page 117: Figure 62. Ion Source Interface Components For The Lxq And Ltq Xl

    Figure 63. Ion source interface components for the Velos Pro Spray cone Vespel O-ring Ion sweep cone Ion transfer tube 3. Clean the O-ring with methanol or replace it if necessary (see page 85). Thermo Scientific LTQ Series Hardware Manual...
  • Page 118 Note If you successfully unblocked the ion transfer tube, the Convectron gauge pressure should increase to a normal value (approximately 1 Torr). If trying this method does not clear the blockage, replace the ion transfer tube. LTQ Series Hardware Manual Thermo Scientific...

  • Page 119: Reinstalling The Ion Source Interface

    2. Carefully insert the ion source interface into the vacuum manifold until it snaps into place. 3. Reinstall the API source housing. For instructions, refer to Chapter 2, “Setting Up the API Source,” in the LTQ Series Getting Started Guide. 4. Start the system as described in “Starting the System after a Complete Shutdown”...
  • Page 120 • Before removing the probe or API source housing, allow the part to cool to room temperature (approximately 20 minutes) before touching it. • If the mass spectrometer connects to an LC system, leave the solvent flow from the LC pump on while the probe cools to room temperature. LTQ Series Hardware Manual Thermo Scientific...

  • Page 121: Removing The Mp00 Rf Lens Assembly

    MP00 rf lens assembly by pulling it toward the left. 5. Using the 5/32 in. hex ball driver, loosen the three hex socket-head screws that secure the ion source interface to the vacuum manifold’s outer cage (Figure 64). Thermo Scientific LTQ Series Hardware Manual...

  • Page 122: Figure 64. Mp00 Rf Lens Assembly Removal

    64) to loosen the outer cage from the vacuum manifold. 7. Carefully remove the MP00 rf lens assembly and place it on the clean, lint-free work surface. 8. Remove the two hex socket-head screws from the ejection screw holes. LTQ Series Hardware Manual Thermo Scientific...

  • Page 123: Disassembling The Mp00 Rf Lens Assembly

    66). If necessary, first loosen the ball plungers by using the small slotted screwdriver. 3. Gently push lens L0 to the back of the outer cage (Figure 66). If necessary, first loosen the ball plungers by using the small slotted screwdriver. Thermo Scientific LTQ Series Hardware Manual...

  • Page 124: Cleaning The Mp00 Rf Lens Assembly Components

    3. Using the 6000 grit MICRO-MESH polishing swabs, clean the bore in each lens. 4. Rinse the components thoroughly with water. 5. Sonicate the components in water for 10 minutes. 6. Sonicate the components in methanol for 10 minutes. LTQ Series Hardware Manual Thermo Scientific...

  • Page 125: Reassembling The Mp00 Rf Lens Assembly

    96). 4. Carefully insert the outer cage assembly into the vacuum manifold. 5. Using the 5/32 in. hex ball driver, tighten the three hex socket-head screws that secure the assembly to the vacuum manifold. Thermo Scientific LTQ Series Hardware Manual...

  • Page 126: Mp0 And Mp1 Ion Guides Maintenance

    Magnification device Gloves, lint- and power-free Phillips screwdriver Graduated cylinder (for use with methanol) Sonicator Kimwipes or lint-free industrial tissues (Optional) Tweezers (or similar tool) Methanol, LC/MS-grade – Nitrogen gas – Water, LC/MS-grade and tap LTQ Series Hardware Manual Thermo Scientific...

  • Page 127: Removing The Top Cover Of The Mass Spectrometer

    3. Disconnect any tubing between the syringe pump or LC system and the API source. 4. Use the 1/4 in. hex driver to loosen the latch on the front door (Figure 67). Figure 67. Front door latch Door latch Thermo Scientific LTQ Series Hardware Manual...

  • Page 128: Figure 68. Top Cover Screws

    (Figure 68). Figure 68. Top cover screws Top cover screws 6. Slide the top cover back and lift it off (Figure 69). Figure 69. Top cover pushed back Top cover LTQ Series Hardware Manual Thermo Scientific...

  • Page 129: Removing The Vacuum Manifold's Top Cover Plate

    PCB cables Handle (front) 2. Use a small slotted screwdriver to loosen the screws that secure the electrometer cable, and then disconnect the cable. 3. Disconnect the three cables connected to the top cover PCB. Thermo Scientific LTQ Series Hardware Manual...

  • Page 130: Removing The Mp0 And Mp1 Ion Guides

    Slide the MP1 support bracket away from MP1 until it disengages from MP1. c. Remove multipole MP1 and the split gate lens and place them on the clean, lint-free work surface. LTQ Series Hardware Manual Thermo Scientific...
  • Page 131 • (For the Velos Pro) Slide both MP0 and the MP0 support bracket away from lens L1 until it disengages from L1. c. Remove MP0 and lens L1 and place them on the work surface. Thermo Scientific LTQ Series Hardware Manual...

  • Page 132: Figure 71. Mp0 And Mp1 Ion Guides For The Lxq (Exploded View)

    Maintenance MP0 and MP1 Ion Guides Maintenance Figure 71. MP0 and MP1 ion guides for the LXQ (exploded view) Multipole support brackets Thumbscrews Split gate lens Lens L1 Baffle Guide post LTQ Series Hardware Manual Thermo Scientific...

  • Page 133: Figure 72. Mp0 And Mp1 Ion Guides For The Ltq Xl (Exploded View)

    Maintenance MP0 and MP1 Ion Guides Maintenance Figure 72. MP0 and MP1 ion guides for the LTQ XL (exploded view) Multipole support brackets Thumbscrews Split gate lens Lens L1 Baffle Guide post Thermo Scientific LTQ Series Hardware Manual...

  • Page 134: Figure 73. Mp0 And Mp1 Ion Guides For The Ltq Velos (Exploded View)

    Maintenance MP0 and MP1 Ion Guides Maintenance Figure 73. MP0 and MP1 ion guides for the LTQ Velos (exploded view) Multipole support brackets Thumbscrews Split gate lens Lens L1 Baffle Guide post LTQ Series Hardware Manual Thermo Scientific...

  • Page 135: Figure 74. Mp0 And Mp1 Ion Guides For The Velos Pro (Exploded View)

    Maintenance MP0 and MP1 Ion Guides Maintenance Figure 74. MP0 and MP1 ion guides for the Velos Pro (exploded view) Multipole support brackets Thumbscrews Split gate lens Lens L1 Baffle Guide post Thermo Scientific LTQ Series Hardware Manual...

  • Page 136: Gate Lens

    10. Using a magnification device, inspect the components for any residual lint or particulates. Note Inspect the inside surfaces and edges to confirm that no lint or particulates are present. Use tweezers or a similar tool to remove the lint or particulate. LTQ Series Hardware Manual Thermo Scientific...

  • Page 137: Reinstalling The Mp0 And Mp1 Ion Guides

    Make sure the contact pin is up and away from the top cover plate. ii. With the other hand, install the MP0 support bracket at the other end of multipole MP0 by lowering and sliding it back toward MP0. Thermo Scientific LTQ Series Hardware Manual...
  • Page 138 (Figure 75 through Figure 77, as applicable). 4. Check all leads to ensure that they are secure and attached to the correct electrodes (Figure 75 through Figure 77, as applicable). LTQ Series Hardware Manual Thermo Scientific...

  • Page 139: Figure 75. Lxq Wiring Diagram For The Mp0 And Mp1 Ion Guides

    Wire 9 (gray) Wire 10 (white) Wire 1 (brown) Wire 8 (violet) Wire 11 (blue) Wire 2 (red) Wire 7 (blue) Wire 12 (violet) Wire 4 (yellow) Wire 5 (green) Wire 6 (black) Wire 3 (orange) Thermo Scientific LTQ Series Hardware Manual...

  • Page 140: Figure 76. Ltq Xl Wiring Diagram For The Mp0 And Mp1 Ion Guides

    Wire 9 (gray) Wire 10 (white) Wire 1 (brown) Wire 8 (violet) Wire 11 (blue) Wire 2 (red) Wire 7 (blue) Wire 12 (violet) Wire 4 (yellow) Wire 5 (green) Wire 6 (black) Wire 3 (orange) LTQ Series Hardware Manual Thermo Scientific...

  • Page 141: Figure 77. Velos Pro Wiring Diagram For The Mp0 And Mp1 Ion Guides

    Wire 9 (gray) Wire 5 (green) Wire 10 (white) Wire 11 (blue) Wire 1 (brown) Wire 12 (violet) Wire 2 (red) Split lens: left (yellow) right (orange) Wire 3 (orange) Wire 6 (black) Wire 5 (green) Thermo Scientific LTQ Series Hardware Manual...

  • Page 142: Reinstalling The Vacuum Manifold's Top Cover

    CAUTION Do not damage the O-ring or the components on the bottom of the top cover plate. 7. Reconnect the following: • Three cables to the top cover PCB (Figure 70 page 101) • One or two electron multiplier HV coaxial cables, as applicable • One electrometer cable LTQ Series Hardware Manual Thermo Scientific...

  • Page 143: Reinstalling The Top Cover Of The Mass Spectrometer

    Note The LXQ mass spectrometer has only one electron multiplier. Adjust the following procedure accordingly. For Field Service use only Part number Electron multiplier, anode, and base shield page 135. Electron multiplier shield retainer (Velos Pro only) 97655-20003 Thermo Scientific LTQ Series Hardware Manual...
  • Page 144 79). Set it aside with the screws. 7. Detach the HV connectors from the HV feedthrough to remove the electron multiplier assemblies. The anode remains in the anode feedthrough in the top cover plate. LTQ Series Hardware Manual Thermo Scientific...

  • Page 145: Figure 78. Electron Multipliers And Dual Anode (Ltq Xl)

    Maintenance Electron Multiplier Replacement Figure 78. Electron multipliers and dual anode (LTQ XL) Electron connector multipliers Anode shield Dual anode Anode feedthrough feedthrough Figure 79. Electron multipliers and shield retainers (Velos Pro) Shield retainer Thermo Scientific LTQ Series Hardware Manual...

  • Page 146: Figure 80. Placement Of Screws For The Left And Right Electron Multipliers (Top View)

    2. Insert the HV connectors into the HV feedthroughs (Figure 80 page 118). Figure 81. Placement of the electron multipliers and shield retainers on the top cover plate Electron multiplier shield retainers LTQ Series Hardware Manual Thermo Scientific...

  • Page 147: Figure 82. Diagnostics Dialog Box Showing The Set Device List

    1. Open the Tune Plus window (see page 34). 2. Choose Diagnostics > Diagnostics to open the Diagnostics dialog box (Figure 82). 3. In the Tools list, select Set Device. Figure 82. Diagnostics dialog box showing the Set Device list Thermo Scientific LTQ Series Hardware Manual...

  • Page 148: Figure 83. Diagnostics Dialog Box Showing The Electron Multiplier Voltage Parameters

    –800 –1300 Multiplier 2 high gain (V) –800 –900 –1200 Multiplier 2 normal gain (V) –800 –800 –1300 c. Click Set. d. Repeat step 5a through step 5c to set the remaining multiplier devices. LTQ Series Hardware Manual Thermo Scientific...

  • Page 149: Figure 84. Diagnostics Dialog Box Showing The Mass Calibration Page

     To save the electron multiplier voltage settings 1. In the Tools list, choose Mass Calibration to open the Mass Calibration page (Figure 84). Figure 84. Diagnostics dialog box showing the Mass Calibration page Thermo Scientific LTQ Series Hardware Manual...

  • Page 150: Figure 85. Calibrate Dialog Box Showing The Automatic Page

    (Thermo Fisher Scientific Product Support recommends 15 hours or more.) 2. Set up for infusing the tuning solution into the mass spectrometer as described in the LTQ Series Getting Started Guide. 3. In the Tune Plus window, click the Calibrate button to open the Calibrate dialog box (Figure 85).

  • Page 151: Figure 86. Semi-Automatic Page In The Calibrate Dialog Box (Lxq And Ltq Xl)

    Maintenance Electron Multiplier Replacement Figure 86. Semi-Automatic page in the Calibrate dialog box (LXQ and LTQ XL) Figure 87. Semi-Automatic page in the Calibrate dialog box (Velos Pro) Thermo Scientific LTQ Series Hardware Manual...

  • Page 152: Forepump Maintenance

    7. Click Start. 8. After the electron multiplier gain calibrations are complete, set up the mass spectrometer for operation as described in the LTQ Series Getting Started Guide. Forepump Maintenance Inspecting, adding, purging, and changing the pump oil are all that is required to maintain the forepump or forepumps, depending on the mass spectrometer model.

  • Page 153: Diagnostics

    If there is a problem with the instrument electronics, the diagnostics can often locate it. Note The diagnostic tests in the Tune Plus application are limited to diagnosing electrical problems only. Thermo Scientific LTQ Series Hardware Manual...

  • Page 154: Figure 88. Diagnostics Dialog Box Showing The General Test Page

    Testing starts and the Testing text box displays a chronological log of all diagnostic tests. After tests are completed for a specific subsystem, the Result column displays either Pass or Fail. If the diagnostics indicates a problem, contact your local Thermo Fisher Scientific field service engineer for assistance. LTQ Series Hardware Manual Thermo Scientific...

  • Page 155: Using The Diagnostic Tools

    3. Click System Evaluation, and then select the check boxes for the evaluation procedures that you want to run. For a description of each system evaluation tool, see System Evaluation Tools. 4. Click Start. The evaluation results appear in the Testing area. Thermo Scientific LTQ Series Hardware Manual...

  • Page 156: System Evaluation Tools

    System Evaluation Tools Table 11 lists the available system evaluation tools (procedures) for the LTQ Series in the order shown in the Tune Plus application. Several of the system evaluation tools operate when the system is in positive or negative mode, and the other tools only operate in positive mode.
  • Page 157 For instructions, refer to Chapter 4 in the ETD Module Hardware Manual. Resonance Ejection Evaluation Determines if the resonance ejection signal is Both functioning by turning it on and off and by observing the resulting change in the spectrum. Thermo Scientific LTQ Series Hardware Manual...
  • Page 158 (LTQ XL, LTQ Velos, and changing the dc offsets of all elements of the trap Velos Pro only) and checking to see if the trap's behavior changes. Available with LTQ Series 2.7 SP1 or later LTQ Series Hardware Manual Thermo Scientific...

  • Page 159: Replaceable Parts

    Replaceable Parts This chapter provides the part numbers for the chemical and accessory kits that come with the LTQ Series mass spectrometers. It also lists the replaceable and consumable parts for each mass spectrometer and their corresponding part numbers. Note Accessory kits for the ETD module and MALDI source are listed in their respective hardware manuals.

  • Page 160: Ms Setup Kit

    APCI Probe Nozzle Assembly ....... . . 97055-60089 LTQ Series Hardware Manual...

  • Page 161: Ion Source Interface Parts

    S-lens ............70005-60182 Thermo Scientific LTQ Series Hardware Manual...

  • Page 162: Mp00 Lens Parts

    Thumbscrews, 10-32 ......... . . 97000-20235 LTQ Series Hardware Manual...

  • Page 163: Electron Multipliers

    (Upchurch Scientific U-435) ........00101-18182 The Velos Pro electron multiplier assembly does not require a separate anode component. Thermo Scientific LTQ Series Hardware Manual...

  • Page 164: Forepumps

    (between outer cage and vacuum manifold) ..... . . 00107-13000 Split chamber O-ring, 12.33 × 6.65 × 3.21 in. (l × w × h) ....97055-40005 LTQ Series Hardware Manual Thermo Scientific...

  • Page 165: Glossary

    The value is normally tuned for a specific compound. API tube-skimmer region The area between the tube and the skimmer, which is surrounded by the tube lens. It is also the area of first-stage evacuation in the API source. Thermo Scientific LTQ Series Hardware Manual...
  • Page 166 API ion transfer tube and acts electrospray ionization (ESI) in combination with as a physical barrier to protect the entrance of the heated auxiliary gas. tube. LTQ Series Hardware Manual Thermo Scientific...
  • Page 167 A multipole rod assembly that is operated with analyzer, and ion detection system. only radio frequency (rf ) voltage on the rods. In this type of device, virtually all ions have stable trajectories and pass through the assembly. Thermo Scientific LTQ Series Hardware Manual...

  • Page 169: Index

    L1 source optics flight time evaluation MP00 rf lens transfer efficiency evaluation skimmer trap linearity evaluation S-lens trap tolerance evaluation split gate lens trap wiring evaluation tube lens system evaluation tools, displaying Communication LED Thermo Scientific LTQ Series Hardware Manual...
  • Page 170 See ion transfer tube functional block diagram ion transfer tube mass spectrometer cleaning vacuum system description fused-silica sample tube removing checking ionization technique, definition for APCI probe, installing for ESI probe installing trimming LTQ Series Hardware Manual Thermo Scientific...
  • Page 171 MP1 ion optics tube lens, cleaning See MP1 ion guide mass analyzer multipole designations xxii description Multipole MP1 helium damping gas See MP1 ion guide operation nitrogen, pressure Thermo Scientific LTQ Series Hardware Manual...
  • Page 172 MP1 ion guide and voltages removing MP1 ion guide and line power specifications resonance excitation rf voltage LTQ Series Hardware Manual Thermo Scientific...
  • Page 173 Index: W WEEE compliance wiring diagrams MP0 and MP1 ion guides, LTQ XL MP0 and MP1 ion guides, LXQ MP0 and MP1 ion guides, Velos Pro Thermo Scientific LTQ Series Hardware Manual...

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