Thermo Scientific Varioskan User Manual page 167

Thermo Fisher Scientific
• ED - Select whether you want an inhibition curve (ED0) or an activity
curve (ED100).
• Use series - Select whether you want to base the effective dose calculation
on Dilution series or on Concentration series.
If you select the Dilution series, the Reference value selection can be
made from Controls and Unknowns. If you select the Concentration
series, the Reference value selection can be made from Controls and
Calibrators.
• Reference value - Select a sample or a replicate, which acts as the ED
or ED value.
0
• Wavelength - Select a desired wavelength for the calculation if multiple
wavelengths were measured.
• Group - Select the groups (assays) for both the controls and the
unknowns used. The calculation is done for all unknown series found
in the selected assay.
• Sample - Select the individual sample.
Thermo Scientific SkanIt
Data Processing Parameters
® ®
Software for Varioskan User Manual
Effective dose
100
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