Trf Decay - Thermo Scientific Varioskan User Manual

TRF decay

Thermo Fisher Scientific
• Dynamic range - Select the dynamic range to be used in the
measurement: AutoRange, Low, Medium Low, Medium High, or High.
For more information, see Dynamic range selections" on page 112.
• Measurement time [ms] - The measurement time defines the light
collection time used in the measurement. The wells are measured with
a selected measurement time. For more information, see Measurement
times" on page 113.
• Lag time [hh:mm:ss.t] - Define the time before the measurement is
executed.
• Step duration [hh:mm:ss] - Define the total time for the step. The
software waits after the instrument has performed the action until the
time is up before proceeding with the following step.
• Optics - Fluorescence can be measured from one position:
• Top - Fluorescence is measured at the measurement position above
the plate.
• Bottom - This option is available with the Varioskan Flash only.
• TRF delay time [µs] - Define the waiting period (TRF delay) between
the excitation flash and the beginning of the emission light measurement
(see Figure 7-1).
• TRF integration time [µs] - Define the length of the measurement
window, that is, the time the emitted signal is collected for each
measurement cycle (see Figure 7-1).
The sum of TRF delay time and TRF integration time can be maximum
2400 µs.
The TRF Decay step is used to determine the life time of the TRF signal.
Select Steps > TRF Decay or click the
Thermo Scientific SkanIt
button on the action panel.
® ®
Software for Varioskan User Manual
Step Parameters
TRF decay
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