Kinetic Loops; Figure 6-3 Interval Time, Total Time And Readings In A Kinetic Loop - Thermo Scientific Varioskan User Manual

Kinetic loops

Thermo Fisher Scientific
Note The readings are numbered in the measurement results so
that the reading where the dispensing is performed is reading no.
0 and the readings before it are given negative numbers. For
example, if the Dispense at reading value is set to 5, the readings
are numbered -4, -3, -2, -1, 0, 1, 2,....
The Lag time and Step duration parameters are not available in the
measurement step.
The kinetic loop step is used for executing the child steps multiple times
before continuing with the protocol, that is, for kinetic measurements. The
kinetic loop can be used with photometric, fluorometric and TRF
measurements. It is also possible to add a dispense step to the kinetic loop
before any of the allowed measurement steps. In this case a well is first
dispensed and then measured immediately before dispensing and measuring
the next well.
You can set the number of readings as well as the interval time. These values
will define the delay between the steps in the loop as well as the total time
of the kinetic loop (Figure 6-3).
Figure 6-3. Interval time, total time and readings in a kinetic loop
When you add a measurement to the kinetic loop, the editing pane shows
the selected measurement step parameters. The kinetic loop parameters can
be edited by selecting the kinetic loop in the Steps step tree.
• Readings - Enter the number of times the kinetic loop is executed. The
default value is 10.
• Interval [hh:mm:ss:t] - Enter the time that elapses between the starting
times of two consecutive loops.
Thermo Scientific SkanIt
® ®
Software for Varioskan User Manual
Protocols
Kinetic loops
91