Nucleic Acid Measurements; Making A Measurement - Thermo Scientific NanoDrop Lite Plus User Manual

1 NanoDrop Lite Plus User Guide

Nucleic Acid Measurements

Nucleic Acid Measurements

Making a Measurement

10 NanoDrop Lite Plus User Guide
Nucleic acid samples can be easily checked for concentration and purity using the
NanoDrop Lite Plus spectrophotometer. To measure nucleic acid samples (dsDNA,
ssDNA and RNA) from the Home screen, select Nucleic Acids and select the
appropriate Nucleic Acid application.
1. From the Home screen, select Nucleic Acids and select the appropriate
sample type.
For DNA measurements, select either the dsDNA or ssDNA assay.
2. Following the on-screen instructions, establish a blank by pipetting 1-2 μL of the
blanking buffer onto the bottom pedestal, lower arm and select Blank.
3. When measurement is complete, raise the arm and wipe the buffer from both the
upper and lower pedestals using a dry laboratory wipe.
4. Measure sample by pipetting 1-2 μL of sample onto the bottom pedestal, lower
arm and select Measure.
5. Wipe the upper and lower pedestals using a dry laboratory wipe and the
instrument is ready to measure the next sample.
Note Use a fresh aliquot of sample for each measurement.
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