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Thermo Scientific EASY-nLC 1200 Manuals
Manuals and User Guides for Thermo Scientific EASY-nLC 1200. We have
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Thermo Scientific EASY-nLC 1200 manual available for free PDF download: Troubleshooting And Maintenance Manual
Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual (311 pages)
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 12 MB
Table of Contents
Table of Contents
11
Preface
17
Related Documentation
17
Safety and Special Notices
18
Contacting Us
20
Chapter 1 Introduction
23
Hardware Components
24
Autosampler
24
Solvent System Components Behind the Right Side Panel
26
Computer and Autosampler Behind the Left Side Panel
37
Back Panel
38
Touch-Screen Application
40
Description of the Touch-Screen Monitor
40
Logging in to the EASY-Nlc Instrument for Maintenance Tasks
43
Closing down the EASY-Nlc Instrument
44
Specifications
45
Performance Specifications
45
Technical Specifications
46
Chapter 2 Maintenance Schedule
49
Daily Maintenance
49
Weekly Maintenance
50
Quarterly Maintenance
50
Half-Yearly Maintenance
51
Yearly Maintenance
52
Field Service Repairs
52
Chapter 3 Maintenance Scripts and Service Records
53
Maintenance Scripts
53
Purge Solvent
54
Flush Air
55
Precolumn Equilibration
56
Analytical Column Equilibration
58
Isocratic Flow
60
Column Conditioning
61
MS Connection
61
Sample Pickup
64
Leaks
66
Pump Torque
69
Back Pressure
69
Autosampler Torque
70
Recording
71
Constant Pressure
72
Constant Flow
73
Flow Sensors
74
Reset Pressure Sensor
76
Direct Infusion
77
Keeping Service Records
79
Checking and Resetting the Device Usage Counters
81
Chapter 4 System Modifications
83
Ordering the UHPLC Liquid Junction Kit
83
Installing the Modified Column out and Waste in Lines
84
Chapter 5 Routine Maintenance
87
Maintaining a Clean Working Environment
88
Using Only UHPLC/MS-Grade Solvents
88
Refreshing Solvents
88
Re-Calibrating Flow Sensors
89
Replacing the Main Power Fuse
90
Maintaining the Syringe Pumps
92
Retracting the Piston
94
Replacing the Piston Seal
95
Priming the Pump
100
Resetting the Pump Usage Counter
102
Removing Air after Replacing a Piston Seal or a Pump
103
Running the Leaks Script after Replacing a Piston Seal or a Pump
103
Replacing the Check Valves
104
Replacing Viper/Nanoviper Solvent Lines
105
Replacing the Autosampler Needle
107
Replacing the Sample Loop
110
Replacing a Pressure Sensor
111
Replacing a Flow Sensor
114
Replacing the Hard Drive
117
Managing the Devices List
121
Chapter 6 Field Service Maintenance
123
Replacing the Built-In Computer
123
Replacing the Monitor
127
Replacing the Autosampler
131
Replacing the Autosampler Cooler
137
Replacing the ASC Cooler
137
Replacing a Rotary Valve
140
Replacing a Pump
143
Replacing a Pump PCB
146
Chapter 7 Troubleshooting
151
Troubleshooting Tips
152
Autosampler Problems
153
Contact Closure Problems
154
Instrument Startup Problems
154
Trapped Air
155
Delayed Elution
155
Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps
161
System Reaches Its Maximum Pressure During the Gradient
163
Sample Signal Weak or Absent
166
Slow or no Pressure Increase in Subsystem a or B
170
Errors Reported by the Xcalibur Data System
173
Carryover
177
Spray Issues
179
Chromatographic Performance
180
Device Failures
181
Miscellaneous
185
Using the Direct Controls for Troubleshooting and Maintenance
187
Using the Valve Controls
187
Using the Pump Controls
188
Using the Autosampler Controls
194
Troubleshooting a Pump that Fails the Flush Air Script
196
Troubleshooting a Pump that Fails the Leaks Script
199
Running a System Leak Test
200
Troubleshooting the Results of the System Leak Test
205
Checking Valve S for Leaks
206
Checking the Sample Loop Connections for Leaks
206
Locating Leaks in the Solvent Path from Valve B to Flow Sensor B
206
Locating Leaks in the Solvent Path from Pump a to Flow Sensor a
208
Locating Leaks in or between Valve S and Valve W
210
Locating Leaks between the Flow Sensors and Valve S
218
Identifying a Leaking Check Valve
229
Troubleshooting a System Blockage
230
Running the Back Pressure Test for the a and B Solvent Paths
231
Troubleshooting a System Blockage When Test Solvent a Fails
232
Troubleshooting a System Blockage When Test Solvent B Fails
238
Troubleshooting the Autosampler Aspiration
242
Checking Sample Pickup
244
Troubleshooting Communication Problems
245
Network Access
245
Network Connection Failures
245
Testing the Network Connection to the EASY-Nlc Computer
247
Verifying that the LC/MS System Is Properly Grounded
249
Chapter 8 Calibrating the Autosampler's XYZ Robot
251
Replacing the Autosampler Adapter Plate
251
Replacing the Adapter Plate in the ASC Model Autosampler
252
Managing Plate Formats
253
Selecting a Plate Format
253
Creating a New Plate Format
254
Deleting a Plate Format
256
Preparing the Autosampler for the Calibration Routines
257
Calibrating Plates
258
Calibrating the Wash Bottle and Extra Vial Positions
263
Chapter 9 Remote Support
265
Connecting the EASY-Nlc Instrument to the Support Server
266
Saving System Files on a USB Removable Storage Device
272
Downloading the Latest Firmware File
274
Chapter 10 Returning the EASY-Nlc Instrument
277
Transport Instructions
277
Appendix A Error Codes
279
Appendix B Mobile Phase Viscosity
287
Appendix C Consumables and Replacement Parts
289
Ordering Information
289
Solvent System Schematic for the EASY-Nlc 1200 Instrument
290
Common Replacement Parts
292
Common Replacement Parts for the EASY-Nlc 1200 Instrument
292
Appendix D Quick Reference Guides for Routine Maintenance
293
Using Nanoviper Fittings Quick Reference Guide
295
Connecting Nanoviper Fittings
295
Nanoviper Tubing Connections
296
Returning the System to Atmospheric Pressure
298
Maintaining the Pump Quick Reference Guide
301
Replacing a Damaged Autosampler Needle
305
Index
307
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