Thermo Scientific gibco CTS Rotea Manuals

Thermo Scientific gibco CTS Rotea Manuals

Manuals and User Guides for Thermo Scientific gibco CTS Rotea. We have 1 Thermo Scientific gibco CTS Rotea manual available for free PDF download: User Manual

Thermo Scientific gibco CTS Rotea User Manual

Thermo Scientific gibco CTS Rotea User Manual (128 pages)

Counterflow Centrifugation System Process Design

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 9 MB

Table of Contents

Table of Contents
3
CHAPTER 1 Product Information

7
CHAPTER 2 Software Installation

12
- Software Installation
  12
CHAPTER 3 Rotea Protocol Builder Application

17
- Create or Open a Protocol
  17
- Define Kit
  18
- CTS ™ Rotea ™ Single-Use Kit Diagram
  19
- Cell Type
  21
- Description
  21
- Kit Type
  22
- Long Description
  22
- Step View
  23
- Define Fluid Path and Direction of Flow
  24
- Step Details
  25
- Step Type
  27
- Trigger
  32
- Volume
  33
- Timer
  35
- Pressure 1 and Pressure 2
  36
- Bubble Sensor Input and Bubble Sensor Output
  37
- Data Entry
  38
- List View
  39
- Step Summary
  40
- Edit Using List View
  42
- Loops
  42
- Repeat Count
  43
- Bubble Sensor Not Triggered
  44
- Process Counter
  44
- Timer (Seconds) and Timer (Minutes)
  45
- Skip Button Triggered
  45
- Editing Loops
  46
CHAPTER 4 Protocol Building Blocks

47
- Priming
  47
- Overview
  47
- Bubbles
  47
- Priming Volumes
  48
- Basic Priming Steps
  49
- Prime Remote Sources
  52
- Manage Air in the System
  53
- Establish a Fluidized Bed
  53
- Overview
  53
- The Seven Parameters for Establishing a Fluidized Bed
  55
- Optimize G Force and Flow Rate
  56
- Cell Size (D)
  56
- Media Density (Ρ)
  57
- Cell Concentration
  57
- Establish a Bed in Recirculation
  57
- Establish a Bed Without Recirculation
  59
- What a Good Bed Looks Like
  60
- Establish a Bed: Troubleshooting
  62
- Cell Loading
  64
- Overview
  64
- Cells Loaded into the CFC Chamber
  64
- Bed Disruption During Loading
  65
- Cell Separation During Loading
  65
- Re-Use Input Bags
  66
- Refill the Bubble Trap
  69
- Connect to Bioreactors
  70
- Wash Cells and Buffer Exchange
  71
- Overview
  71
- Media Selection
  72
- Wash to Similar Density Media
  72
- Wash to more Dense Media
  72
- Wash to Lower Density Media
  73
- Defensive Washing Strategies
  73
- Elutriation
  77
- Overview
  77
- Improve Cell Viability
  79
- Leukopak Processing
  79
- Typical Leukaphoresis Composition
  80
- Lentivirus Clarification
  81
- Separate Cells from Microcarriers
  81
- Elutriation Techniques
  82
- Concentrate Recovery
  85
- Overview
  85
- Normal Step
  86
- Speed Settings
  86
- Pause Step
  87
- Harvest Step
  88
- Harvest Using OD Sensor
  90
- Multi-Bite Processing
  92
- Overview
  92
- Determine the Number of Cells Per Bite
  92
- Maximize Throughput
  93
- Process Integrity
  94
- Overview
  94
- CTS ™ Rotea ™ Single-Use Kit
  94
- Check Manual Clamps
  95
- Verify Reagent Connected and Not Clamped
  95
- Check for Closed Clamps
  95
CHAPTER 5 Rotea

96
- Process Model
  96
- Counter Flow Centrifugation Forces
  96
- Predict Process Settings
  98
- Select and Format Media
  100
- Create Custom Media
  102
- Model Particle Behavior
  103
- Change and Delete Particles from a Model
  106
- Create Custom Particles
  107
- Edit Custom Particles and Custom Media
  107
CHAPTER 6 Rotea

109
- Simulation Software
  109
- Rotea ™ Simulation Software
  109
CHAPTER 7 Design New Protocols

115
- Plan the Kit Configuration
  115
- Plan the Protocol
  115
- Prepare for the First Trial Run
  116
- Water Runs
  116
- First Run with Cells
  116
- Analyze the Outcome
  116
- Refine the Protocol to Achieve Target Cell Outcomes
  116
- Add Process Integrity Features
  117
APPENDIX A Safety

118
- Symbols on this Instrument
  118
- Standard Safety Symbols
  119
- Control and Connection Symbols
  119
- Conformity Symbols
  119
- Safety Information for Instruments Not Manufactured by Thermo Fisher Scientific
  121
- Instrument Safety
  121
- General
  121
- Physical Injury
  122
- Electrical Safety
  122
- Cleaning and Decontamination
  123
- Safety and Electromagnetic Compatibility (EMC) Standards
  123
- Safety Standards
  123
- EMC Standards
  124
- Environmental Design Standards
  124
- Chemical Safety
  125
- Biological Hazard Safety
  126
APPENDIX B Documentation and Support

127
- Customer and Technical Support
  127
- Limited Product Warranty
  127

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