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D.1 UV Cutoff Wavelengths of Common Mobile Phases
The mobile phase composition affects its UV cutoff (the minimum effective
wavelength for the measurement). In general, mobile phases are solvents such as
water, acetonitrile, or methanol. They may also contain salts, such as sodium
hydroxide.
The UV cutoff wavelengths of these solvents may differ from the predicted values
listed in
Table
the solvent affect the UV cutoff. Therefore, the values listed in
approximate values only. The cutoffs listed in the table apply to spectro-quality
grade solvents.
Solvent
Acetic acid
Acetone
Acetonitrile
Dioxane
Ethanol
Ethyl acetate
Hexane sulfonic acid (0.005 M)
Isopropanol
Methanol
Methylene chloride
n-Hexane
Octane sulfonic acid
Sodium carbonate (0.01 M)
Sodium hydroxide (0.1 M)
Tetrabutylammonium hydroxide
(0.005 M)
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D-1. Among other factors, the degassing quality and purity grade of
Table D-1. Properties of Common Mobile Phases
D • UV Absorbances
UV Cutoff
Refractive
(nm)
Index
208
1.370
330
1.356
190
1.341
215
1.420
210
1.359
256
1.370
230
205
1.384
205
1.326
233
1.421
190
1.372
230
210
217
215
Table D-1
are
Selectivity
Group
IV
VIa
VIb
VIa
II
VIa
II
II
V
VII
109
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