Introduction; Data Types; Ion Polarity Modes - Thermo Scientific TSQ Series Hardware Manual

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Introduction

TSQ™ Series mass spectrometers are members of the Thermo Scientific family of mass

spectrometers. The TSQ Series mass spectrometer is an advanced analytical instrument that

includes a syringe pump, a divert/inject valve, a mass spectrometer, and the Xcalibur™ data system.

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In a typical analysis, a sample can be introduced in the following ways:

• Using the syringe pump (direct infusion)

• Using the divert/inject valve fitted with a sample loop and LC (flow injection analysis)

• Using the divert/inject valve and HPLC fitted with a column (LC/MS)

In a typical analysis, a liquid chromatograph (LC) introduces a sample. The LC separates the

sample into its various components. The components elute from the LC and pass into the mass

spectrometer where they are analyzed.

Analysis by direct infusion or flow injection provides no chromatographic separation of

components in the sample before they pass into the mass spectrometer. The data from the mass

spectrometer is then stored and processed by the data system.

The TSQ mass spectrometer consists of an atmospheric pressure ionization (API) source, ion

optics, triple-stage mass analyzer, and ion detection system. A vacuum manifold encloses the ion

optics, mass analyzer, ion detection system, and part of the API source. Ionization of the sample

takes place in the API source. The specific process used to ionize the sample is known as the

ionization mode. The ion optics transmit the ions produced in the ion source into the mass

analyzer, where they are separated according to their mass-to-charge ratio. The polarity of the

potentials applied to the lenses in the ion source and ion optics determines whether they transmit

positively charged ions or negatively charged ions to the mass analyzer. You can configure the TSQ

mass spectrometer to analyze positively or negatively charged ions (called the positive or negative

ion polarity mode).

Thermo Scientific

Ionization Modes

Scan Modes

Scan Types

Mass/Charge Range

TSQ Series Hardware Manual

Table of Contents

Table of Contents