Practical Tips For Different Types Of Items - Thermo Scientific VARIOKLAV 25T Operating Manual

Rack system
The rack system is designed to accommodate up to 2 or 4 insert trays within
the chamber by simple turning by 90°.
Insert trays
Trays are well suited for assembling and transporting the items to be
sterilized. The steam easily penetrates the perforated tray floor. No
condensate collects in the trays.

5.2.2 Practical tips for different types of items

These practical tips are suggested for different types of items when loading the
sterilizing chamber (8):
Glassware:
♦ First, remove all coarse soiling.
♦ Place all vessels in baskets so that the openings face downwards.
♦ Concave and convex parts should be tilted so that condensate can run
off.
♦ Select the Instruments program.
Pipette tips:
If anchored boxes are used for storing pipette tips, these must be permeable
to steam. Boxes must not be stacked on top of each other. Pipette tips must
not be sterilized in closed boxes. Pipette tips will be moist following
sterilization.
♦ Select the Instruments program.
Destruction (Waste)
INFORMATION
Only sterilize waste in open vessels (e.g. metal boat with
closed bottom).
♦ Place the waste in open vessels or bags or a metal tray with a solid
bottom.
Place open bags additionally into a metal boat with closed bottom. In this
way, you can avoid soiling the sterilizing chamber (8) in the event that the bag
tears open or leaks.
Do not sterilize waste in large destruction bags. The temperature in the
destruction bag can be up to 10 °C lower than the chamber temperature.
You should select the highest possible sterilizing temperature. More heat will
result in better sterilization. As a rule, it is sufficient to set a sterilizing time of
20 minutes at 134 °C.
♦ Select the Destruction (Waste) program.
For predominantly liquid waste:
♦ Select the Liquids program.
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