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4.
(Optional) Adjust the duration of the gel run using the
+/– buttons or press in the duration field to open a
keyboard to enter a number.
5.
Press Start run to begin running the gel.
Note: Do not exceed the maximum run time indicated
for the specific gel type, as this will impact separation
quality.
6.
The run stops automatically after the programmed time
has elapsed and beeps.
a.
b.
7.
Proceed to image capture (see page 20) or other
downstream application.
Check status
The status and the remaining run time of the protocol are indicated on the status dial.
DNA separation can be viewed in real time by turning on the transilluminator. This feature is only
compatible with gels containing dyes visible by blue light transillumination (i.e., E-Gel
SYBR
Safe, E-Gel
™
For optimal viewing, dim the ambient lighting in the room, or use the E-Gel
for visualization (see page 22).
View gel
1.
Press Back light to activate the blue light
transilluminator.
Note: The transilluminator turns off automatically after
1 minute.
2.
Monitor the sample in real-time during the run.
3.
Press Back light again to switch off the blue light
transilluminator.
View gel
Important! Always wear Safe Imager
opened.
with filter
lid open
The transilluminator turns off automatically when the filter lid is opened.
Press Back light to re-activate the blue light transilluminator.
Modify a run
The E-Gel
the duration to exceed the maximum allowable run time for the specific E-Gel
Pause the
1.
Press Pause run to temporarily stop the run.
run
2.
Press Resume to restart the run.
18
Press More time to run the gel longer.
Press Done to end the protocol.
CloneWell
II, E-Gel
™
™
protocol can be cancelled or modified during the run. however the device does not allow
™
and SizeSelect
II agarose gels).
™
™
Viewing Glasses when viewing the gel with the filter lid
™
E-Gel
Power Snap Electrophoresis System User Guide
™
EX, E-Gel
™
™
Power Snap Camera
™
protocol.
™
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Table of Contents
Troubleshooting
