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C.3 Programming Examples
Examples of different pipetting sequences are described below, and are
ideal for use as training exercises. Press the keys shown in keycaps [ ],
then press the [Vol] key until the volume in parentheses ( ) is displayed. At
the end of the program, a triple beep tone
Recommendations for Accurate Pipetting for suggestions for better precision
and accuracy).
C.3.1 Sample Transfers
Example: Fill and dispense 100µl.
s
Vol
[Fill]
(100) [Enter]
t
s
Vol
[Disp]
(100) [Enter] [Enter]
t
The second option is a one-step program. Because the program ends at a
volume greater than zero, and the last step is not a mix, a "Purge" prompt
automatically appears at the end of the fill to dispense the liquid and return to
the beginning of the program.
C.3.2 Incremental Pipetting
Example: Fill and dispense 250 µl.
Option 1
s
Vol
[Fill]
(250) [Enter]
t
s
Vol
[Disp]
(10) [Enter]
t
s
Vol
[Disp]
(45) [Enter] [Enter]
t
Programming and Operation
is sounded. (see section D.3
Option 2
s
Vol
[Fill]
(100) [Enter] [Enter]
t
Note: The 10 µl Primer
Dispense reduces the
percentage of error in
incremental dispensing
(see section D.3
Recommendations for
Accurate Pipetting)
19
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