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KingFisher Pure RNA Blood Kit
Thermo Scientific KingFisher Pure RNA Blood Kit Manuals
Manuals and User Guides for Thermo Scientific KingFisher Pure RNA Blood Kit. We have
1
Thermo Scientific KingFisher Pure RNA Blood Kit manual available for free PDF download: Instruction Manual
Thermo Scientific KingFisher Pure RNA Blood Kit Instruction Manual (36 pages)
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 1 MB
Table of Contents
Table of Contents
3
Chapter 1 Kit Content
5
Kit Content
5
Additional Reagents Required
6
Storage Conditions
6
Chapter 2 Product Description
9
Intended Use
9
Introduction
9
Product Description
9
Kit Specifications
10
Principle and Procedure
10
Kingfisher Magnetic Particle Processors
11
Chapter 3 Safety Information
13
Safety Information
13
Chapter 4 Storage Conditions and Preparation of the Reagents
15
Preparation of the Dnase I Storage Solution
16
Preparation of the Dnase I Working Solution
16
Starting Material Handling and Storage
16
Chapter 5 Protocols and Pipetting Instructions
17
Instructions for Kingfisher Flex with 96 Deep Well Plates
18
Instructions for Kingfisher Duo with 12-Pin Magnet Head
20
Instructions for Total RNA Purification from Paxgene Stabilized Blood Samples
23
Instructions for Total RNA Purification from Tempus Stabilized Blood Samples
24
Quantification and Determination of the Purity of RNA
24
Binding, Wash, and Elution Steps
27
Preparation of the Lysis Buffer
15
Preparation of the Wash Buffers and Rebinding Buffer
15
Storage Conditions
15
Avoiding Ribonuclease (Rnase) Contamination
17
Handling of Kingfisher Magnetic Beads
17
Chapter 6 General Information
27
General Information
27
Handling of Magnetic Beads
27
Reagent Specificity and Volumes
27
Decontamination and Disinfection of Sample Material
28
Appendix A Troubleshooting
29
Appendix B Typical RNA Yields
31
Appendix C Ordering Information
33
Thermo Fisher Scientific Thermo Scientific Kingfisher Pure RNA Blood Kit
35
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