System Reaches Its Maximum Pressure During The Gradient - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

System Reaches Its Maximum Pressure During the Gradient

Table 16. Possible causes of maximum pressure spikes during a run
Symptom
The system reaches its
maximum pressure during a
run.
Thermo Scientific
If the pressure trace shows maximum pressure during the gradient, review the possible causes
in Table 16.
Possible cause
Clogged column
Clogged system
Excessively high flow rate
When a column or the Column Out line is clogged, the backpressure can increase to the
maximum system pressure for pump A, pump B, or pumps A and B during the gradient
(see Figure 120).
Figure 120. Backpressure shown reaching the maximum system pressure for pumps A and B
Action
Run the Back Pressure script for pumps A and B.
If the test passes, one of the columns is probably
clogged and needs to be replaced.
Run the Back Pressure script for pumps A and B.
If the test fails, go to
Blockage" on page
Reduce the flow rate in the method.
Maximum pressure
reached for pump A
EASY-nLC 1200 Troubleshooting & Maintenance Guide
7
Troubleshooting
Troubleshooting Tips
"Troubleshooting a System
208.
Maximum pressure
reached for pump B
141