Troubleshooting The Autosampler Aspiration - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

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Troubleshooting

Troubleshooting the Autosampler Aspiration

Troubleshooting the Autosampler Aspiration
220 EASY-nLC 1200 Troubleshooting & Maintenance Guide
Troubleshooting the autosampler aspiration requires these tools and materials:
Tools
❖ To troubleshoot the autosampler aspiration of sample into the needle
1. Make sure that the W3 wash bottle contains sufficient solvent.
2. Use the Flush Air script to remove air from pump S as follows:
a. Press Maintenance > Scripts.
b. In the Category list, select Prepare.
c. In the Name list, select Flush Air.
d. Press the Parameters tab.
e. Select the Flush Pump S check box.
f. For Flush Threshold [µL], 12 (see
Figure 164. Flush Air script
Parts and materials
• Powder-free gloves
• Sample vial filled with solvent A
• Microtiter plate (if you are using a microtiter plate
format)
Figure 164).
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