Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 59

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2. Set up the parameters for this script as follows:
a. Press the Parameters tab.
b. In the Volume [µL] box, enter the volume of solvent A to use to equilibrate the
analytical column.
For best results, use at least 10 column volumes to equilibrate the analytical column.
c. In the Flow box [µL/min], enter the flow rate for the analytical column equilibration
step.
If the flow field is left empty, the pump operates at the set pressure.
d. In the Max Pressure [bar] box, enter the maximum allowed pressure.
If the pressure field is left empty, the pump operates at the set flow (as long as it is
below the instruments maximum pressure of 1200 bar).
If both a flow and a maximum pressure are specified, the pump flow is limited by
whichever parameter is reached first.
CAUTION Check the maximum pressure rating for the analytical column.
Running the instrument at pressures higher than the maximum pressure rating
for the column reduces the column lifespan.
3. Press Start.
3
Maintenance Scripts and Service Records
EASY-nLC 1200 Troubleshooting & Maintenance Guide
Maintenance Scripts
37