Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 202

7
Troubleshooting
Running a System Leak Test
180 EASY-nLC 1200 Troubleshooting & Maintenance Guide
2. Depending on the results of the Flush Air script displayed on the Output page, do the
following:
• If the script passes, run a system leak test as described in the next procedure,
a system leak test."
• If one of these messages appears, go to
Air Script" on page
– Aborting unable to build pressure
– Aborting check solvent level
CAUTION Wear powder-free gloves and safety glasses when handling parts of the LC
system that come into contact with solvents.
❖ To run a system leak test
1. If you have not already done so, run the Flush Air script to prepare the instrument for a
system leak test (see the previous procedure
test).
2. Connect the Column Out and Waste In lines with the appropriate union (see
page 179).
Note The EASY-nLC 1200 instrument ships with Column Out and Waste In lines
that have nanoViper fittings at both ends. However, if you have modified the
instrument to use columns that have bare fused-silica ends, these solvent lines do not
have fittings on their free ends.
For an EASY-nLC 1200 instrument with the standard nanoViper Column Out and
Waste In lines, do the following:
a. Insert the nanoViper fittings on the ends of the Column Out and Waste In lines into
the Viper union.
b. Alternate tightening each fitting in small increments until you feel resistance. Then
Tighten both fittings by an additional 45 degrees (1/8-turn). Do not tighten the
fittings by more than a 1/4-turn (see
page 83).
CAUTION Because the fittings seal against each other inside the union, do not
tighten them by more than 90 degrees (1/4-turn). Overtightening the fittings can
irreparably damage their sealing surfaces.
Figure 143 shows the connection between the standard Column Out and Waste In
lines. The Viper union makes a zero-dead-volume connection between the two
nanoViper fittings.
"Troubleshooting a Pump That Fails the Flush
174.
To prepare the instrument for a system leak
"Replacing Viper/nanoViper solvent lines"
"To run
Table 25 on
on
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